谷子窄颖花突变体sins1的表型分析和突变基因定位

Morphological Characterization and Gene Mapping of sins1(narrow spikelet 1) in Foxtail Millet

利用甲基磺酸乙酯(EMS,ethyl methyl sulfonate)诱变剂处理野生型Yugu1(豫谷1号),在后代中发现了一个可以稳定遗传的颖花明显变窄的突变体,将其命名为sins1。与Yugu1相比,突变体sins1的株高显著降低了3.89%,穗长和穗粗分别显著降低了17.42%和21.62%,旗叶叶长和叶宽分别显著降低了15.09%和25.78%,千粒重显著降低了40.96%,谷码数显著降低了25%,均达到显著水平(P<0.05)。利用突变体sins1为母本、SSR41为父本构建F_2定位群体,F_2正常颖花与窄颖花植株数目的分离比例为3∶1,表明该突变性状由隐性单基因控制。利用F_2群体隐性单株,最终将突变基因定位在3号染色体上SSR标记3-2658与CAAS3031间约7.709 Mb的距离内,为下一步精细定位提供了基础,同时也为促进禾本科作物颖花的研究提供了方向。

A foxtail millet narrow spikelet mutant which was induced from Yugul by EMS treatment, was ge- netically identified and named as sinsl. The mutant exhibited narrow-spikelet, low-plant-height, short-thin-panicle, short-narrow-leaf, low-spikelet-number per panicle, as well as low-1000-grain weight. Compared with its wild-type Yugu 1, plant height, panicle length, panicle diameter, flag-leaf length, flag-leaf width, the 1000-grain weight, spikelet number per panicle of sinsl were decreased by 3.89% ,17.42% ,21.62% ,15.09% ,25.78% ,40.96% ,25% re- spectively（P 〈 0.05 ）. Genetic analysis showed that 3 ： 1 segregation ratio of wild type to mutant plants were ob- served in the F2 generation of sinsl x SSR41, suggesting that the narrow-spikelet trait of sinsl mutant was controlled by a single recessive nuclear gene. Genetic mapping of the mutant gene was conducted using narrow-spikelet indi- viduals from the F2 segregating population of sinsl ~ SSR41. Finally ,the mutant gene was mapped into genomic re- gion within SSR markers 3-2658 and CAAS3031 in chromosome 3 ,with a 7. 709 Mb interval. This research had laid the foundation for fine-mapping of the narrow spikelet causal gene and will promote spikelet development related re-search in cereal crops.