艾塞那肽抑制子宫内膜癌Ishikawa细胞株裸鼠移植瘤的血管生成

Exenatide Weaks Tumor Angiogenesis within Endometrial Cancer Ishikawa Xenografts in Nude Mice

【目的】探讨艾塞那肽在子宫内膜癌细胞株Ishikawa裸鼠皮下移植瘤内血管生成中的作用。【方法】构建子宫内膜癌细胞株Ishikawa裸鼠皮下移植瘤动物模型;实验分为艾塞那肽组和对照组;采用免疫组化的方法检测瘤内微血管密度(MVD,CD31阳性)和巨噬细胞浸润密度(MID,F4/80阳性);采用Western blot检测瘤内的血管生成因子(VEGF)的含量。【结果】(1)艾塞那肽组瘤内MVD-CD31阳性为(13.2±1.4)/400高倍镜,比对照组的(25.9±5.8)/400高倍镜少(P<0.01)。(2)艾塞那肽组的瘤内VEGF含量低于对照组(P<0.05)。(3)艾塞那肽组瘤内MID-F4/80阳性为(31.4±3.4)%,比对照组的(72.1±4.2)%低(P<0.01)。【结论】艾塞那肽可减少子宫内膜癌Ishikawa细胞株裸鼠移植瘤局部的肿瘤相关巨噬细胞的浸润,减少VEGF生成,抑制移植瘤内的血管生成。

[ Objective ] To investigate the role of exenatide on angiogenesis in endometrial cancer Ishikawa xenografts in nude mice. [ Method ] We used the subcutaneous human endometrial cancer cell Ishikawa xenografts in nude mice model, and divided them into control group and exenatide-treated group. The harvested tumors were preserved for immunohistochemistry staining and western blot analysis. [ Results ] The micro-vessel density （CD31 positive） in exenatide-treated group was （13.2 ± 1.4）/400 power field, less than that in control group [ （25.9 ± 5.8）/400 power field ] （P 〈 0.01 ）. The expression level of VEGF was significantly lower in exenatide-treated group than that in control group （P 〈 0.05 ）. The mean density of tumor associated macrophages （F4/80 positive） is （31.4 ± 3.4）% in exenatide-treated group and （72.1 ± 4.2）% in control group with significant difference （P 〈 0.01 ）. [ Conclusion ] Exenatide weaks tumor angiogenesis within endometrial cancer Ishikawa xenografts in nude mice.