肥猪散中制何首乌的薄层鉴别方法研究

Polygoni Multiflori Radix Praeparata by TLC Identification in Feizhu San

建立了肥猪散中制何首乌的薄层色谱(TLC)定性鉴别法。以制何首乌为对照药材,采用甲苯-乙酸乙酯-甲酸(15∶2∶0.8)为展开剂,在UV365 nm波长下检视,氨蒸气为显色剂,对肥猪散中制何首乌进行薄层色谱法鉴别。结果显示制何首乌斑点清晰,阴性无干扰。本方法简便、快速、准确、重现性好,可用于该制剂的质量控制。

To establish a method of thin layer chromatography （ TLC ） identification for Polygoni Multiflori Radix Praeparata in Feizhu San. Using Polygoni Multiflori Radix Praeparata as contrast medicinal material, methylbenzene-ethyl acetate-methanoic acid（ 15 ： 2 ： 0.8） as developing reagent, inspected in ultraviolet light （365 nm）, ammonia vapor as color developing rengent, to carry out experiment of TLC for Polygoni Multiflori Radix Praeparata in Feizhu San. The TLC chromatograms of Polygoni Muhiflori Radix Praeparata is not only feasible with clear spots, good separation, and no interference.The method is simple, quick, accurate, reproducible and can be used for the quality control of Feizhu San