基于GC-MS的浒苔代谢产物提取方法研究

Metabolite Profiling of Seaweed Enteromorpha Using Gas Chromatography-Mass Spectrometry

[目的]研究浒苔代谢产物的提取方法。[方法]以浒苔为研究对象,用不同的细胞破碎方式、溶剂提取体系、料液比等对均一样本的代谢产物进行提取,气相色谱-质谱法(GC-MS)鉴定代谢产物成分,利用主成分分析等方法,对不同的代谢提取体系进行评价。[结果]溶剂体系甲醇-三氯甲烷-水(7∶3∶3)和液氮处理-球磨破碎-超声波的破壁方法提取并鉴定出81种代谢产物,该方法具有高提取产率、高重现性、易操作和速度快等优点。[结论]溶剂体系甲醇-三氯甲烷-水(7∶3∶3)和液氮处理-球磨破碎-超声波破壁是适合浒苔代谢物提取的方法,该方法可为浒苔及大型绿藻的代谢组学研究提供参考。

[ Objective] To study the extraction method of Enteromorpha metabolites. [ Method] Enteromorpha as the research object, using different cell crushing methods, solvent extraction system, solid-liquid ratio, the homogeneous sample metabolites were extracted; the metabolites were identificated by GC-MS; using principal component analysis method, different metabolic extraction systems were evaluated. [ Result ] A total of 81 metabolites were extracted and identified by the solvent system of methanol： chloroform： water （7： 3：3 ） and liquid nitrogen treatment- milling-ultrasonic breaking method, showing high extraction yield, high reproducibility, easy operation and fast and other characteristics. [ Conclusion] It is a suitable method for extracting metabolites of Enteromorpha which can provide reference for metabonomics study of Enteromorpha and large green algae.