四神丸对溃疡性结肠炎大鼠血清相关炎性因子表达及结肠组织病理形态的影响

Effects of Sishen Wan on the expression of associated inflammatory factors of serum in rats with ulcerative colitis

目的研究四神丸对溃疡性结肠炎(UC)大鼠血清白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)和细胞信号转导抑制因子3(SOCS3)表达及结肠组织病理形态的影响。方法将SPF级Wistar大鼠随机分为空白组、模型组、美沙拉嗪组及四神丸组4组,采用复合方法复制脾肾阳虚型UC大鼠模型。各组大鼠以相关药物及生理盐水灌胃21 d后处死,取大鼠结肠,肉眼观察结肠黏膜大体形态损伤并进行评分;分离各组大鼠血清,采用ELISA法检测血清IL-2,TNF-α,SOCS3的表达水平。结果与空白组比较,模型组大鼠结肠黏膜大体形态损伤评分及血清IL-2,TNF-α水平显著升高,血清SOCS3水平显著降低,差异均有统计意义(P<0.05);与模型组比较,美沙拉嗪组与四神丸组大鼠结肠黏膜大体形态损伤评分及血清TNF-α水平显著降低,血清SOCS3水平显著升高,差异均有统计意义(P<0.05)。结论四神丸可能通过调节血清TNF-α,S OCS3的水平来调控肠上皮免疫系统,从而达到治疗UC的目的。

Objective To explore the effects of Sishen Wan on the expression of serum interleukin 2 （IL-2）, tumor necrosis factor-α pathological forms of colonic tissue in model （TNF-α） and suppressor of cytokine signaling rats with ulcerative colitis （UC）. Methods hypothalamus cytokine 3 （ SOCS3 ） as well as SPF Wistar rats were randomly divided into control group, model group, mesalazine group and Sishen Wan group. UC rat models were re- produced by complex method. All rats were given drugs and saline by garage for 21 days. Then microscopic da- mage on colonic mucosa was observed. The levels of IL-2, TNF-α and SOCS3 in serum were detected by ELISA. Results Compared with control group, the macroscopic damage scores, the levels of serum IL-2 and TNF-α in model group increased ,while SOCS3 decreased obviously（ P〈0.05 ） ; compared with model group ,the macroscopic damage scores and the levels of serum TNF-α decreased, while SOCS3 increased in mesalazine group and Sishen Wan Group. The differences were all significant （P〈0.05）. Conclusion Sishen Wan can cure UC by adjusting the levels of TNF-α and SOCS3 factors to regulate and control the immune system of intestinal epithelium.