抗锶放线菌的分子鉴定及对Sr^(2+)的吸附效果和初步吸附机理分析

Molecular Identification, Adsorption Effect and Preliminary Adsorption Mechanism of Strontium Resistant Actinomycetes

从大亚湾核电站附近水体中分离筛选出一株抗锶放线菌(编号YF-64),研究了该菌株在不同环境参数条件下对锶的吸附效果和初步机理,通过形态和16S rDNA序列相结合的方法对菌株进行了分类鉴定,采用红外光谱(FT-IR)分析技术对菌株吸附前后进行表征,探讨其吸附机制。结果表明,抗锶菌株隶属于天蓝黄链霉菌(基因序列登录号:JF901702),菌株对Sr^(2+)具有较强的吸附效果,根据数据显示,当接触时间为50 min,pH值为6,Sr^(2+)初始浓度为50 mg/L,摇床转速为120 r/min时,该菌株的吸附效果达到最佳为44.29 mg/g;FT-IR结果显示,JF901702菌株对Sr^(2+)的吸附主要是由细胞壁上的羟基,次甲基,羰基起主要吸附作用。由此可知,JF901702菌株可作为经济、高效、环境友好的生物吸附材料进行废水重金属处理。

In this paper, a strontium resistant actinomycetes （numbered YF-64） was isolated from water area near Daya Bay nuclear power station, and adsorption effects and preliminary mechanism of the strain on Sr at different environmental parameters were studied. The strain was identified on the basis of morphology combined 16S rDNA sequence analysis. Infrared spectral analysis technology was used to characterize the stain before and after the biosorption and the adsorption mechanism was discussed. The results indicated that the strain YF-64, identified as Streptomyces coelicoflavus （Gene sequence registration number： JF901702） had a comparatively strong biosorption ability against strontium. The experimental data revealed that under the optimum conditions that pH was 6.0, with contact time of 50 min, initial Sr^2＋ concentration of 50 mg/L and rotation rate at 120 r/min, the biosorption capacity reached to the best as 44.29 mg/g. The Fourier transform infrared spectroscopy （FT-IR） analysis showed that the main functional groups responsible for biosorption of strontium were hydroxyl, methylene and carbonyl on the cell walls. Thus, it was concluded that JF901702 strain could be used as an economic, efficient, environment-friendly material for removal of heavy metals such as strontium from wastewater.