南极磷虾粉对小鼠骨质疏松性骨折愈合的影响

Effects of Antarctic krill meal on fracture healing in osteoporotic mice

目的观察南极磷虾粉(Antarctic krill meal,AKM)对小鼠骨质疏松性骨折愈合过程的影响。方法对10周龄雌性C57BL/6小鼠行双侧卵巢切除术建立骨质疏松模型,3个月后建立右胫中上1/3处开放性骨折模型。随后随机分为4组:一般性骨折组(Sham)、骨质疏松性骨折模型组(OVX)、阳性对照组(ALN,1mg/kg·BW)、AKM组(800mg/kg·BW)。于骨折后5、11、24、35d进行取材,观察AKM对小鼠血清生化指标(VEGF、BALP)及骨痂组织形态学的影响,并探讨AKM促进小鼠骨质疏松性骨折愈合的机制。结果AKM能显著提高血清中VEGF、BALP的水平,促进软骨性骨痂向骨性骨痂转化,加速骨折愈合。AKM能显著促进骨折愈合过程中促血管生成因子(Ang1、PDGF-BB、CD31)、软骨基质形成及降解Marker(Aggrecan、MMP-13)、骨形成相关因子(BMP-2、Col1α1、OCN、TGF-β1)的mRNA表达,加速软骨内骨化。结论 AKM能通过促进软骨内骨化而加速小鼠骨质疏松性骨折愈合进程。

Objective To investigate the effects of Antarctic krill meal on fracture healing in ovariectomy induced osteoporotic mice.Methods The ten-week mice were subjected to bilateral ovariectomy,and subsequently tibia fracture operation after three months to establish an osteoporotic fracture model.All fractured mice were divided into four groups：general fracture group（Sham）,osteoporotic fracture model group（OVX）,alendronate positive model group（1mg/kg·BW）and AKM group（800mg/kg·BW）.Serum biochemical indactors,callus histomorphology and key genes in fracture healing were investigated.Results AKM significantly improved the levels of VEGF and BALP in serum and promoted the transformation of cartilage callus to osseous callus,thereby facilitating fracture healing.Mechanism research revealed that AKM significantly promoted endochondral ossification via increasing the mRNA expression of angiogenesis factor（Ang1,PDGF-BB,CD31）,cartilage matrix formation and degradation Marker（Aggrecan,MMP-13）and bone formation indactors（BMP-2,Col1α1,OCN,TGF-β1）,Conclusion AKM could facilitate fracture healing in osteoporotic mice via promoting endochondral ossification.