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疏肝健脾方对腹泻型肠易激综合征模型大鼠结肠神经生长因子及其受体影响 被引量:5

Effects of Shuganjianpi prescription on the colon mucosa nerve growth factor and its receptors in diarrhea-predominant irritable bowel syndrome model rats
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摘要 [目的]观察和分析中药疏肝健脾方对腹泻型肠易激综合征(IBS-D)模型大鼠结肠神经生长因子(NGF)及其受体TrkA、P75表达的影响,以期阐述中药疏肝健脾方治疗IBS-D可能机制。[方法]将18只2日龄SD雄性幼鼠随机分为正常组、模型组、疏肝健脾组,除正常组外其余各组使用母婴分离加束缚应激的方法制备IBS-D大鼠模型。造模成功后正常组、模型组予生理盐水,疏肝健脾组予疏肝健脾方灌胃14d。取大鼠结肠黏膜,采用酶联免疫法检测NGF及其TrkA、P75表达。[结果]疏肝健脾方可以降低IBS-D模型大鼠结肠黏膜组织NGF含量(P>0.05)、5-HT含量(P<0.05),下调受体TrkA、P75的表达(P<0.05)。[结论]疏肝健脾方干预IBS-D模型大鼠,改善内脏敏感性及腹泻部分机制与调节NGF及其TrkA、P75表达有关。 [Objective]To investigate and analysis the effects of Shuganjianpi preseription(SGJP)on the expression of nerve growth factor(NGF), Trk A receptor and P75 receptor of colon mucosa in the irritable bowel syndrome-diarrhea predominant (IBS-D)model rats. [Methods] Eighteen 2-day-old male Sprague- Dawley(SD) rats were randomly divided into three groups:normal group, model group, and SGJP group. All groups except the normal group received neonatal maternal separation plus restraint stress for preparation of IBS-D model,which were intragastric administrated with saline and SGJP for 14 days respectively after modeling successfully. The colon mucosa of rats was taken for detecting the expression of NGF, Trk A re- ceptor and P75 receptor by enzyme linked immunosorbent assay. [Results]The SGJP reduced the expression of NGF(P〉0.05),TrkA receptor(P〈0.05)and P75 (P〈0.05)receptor of colon mucosa in IBS-D rats. [Conclusion]The partial mechanisms of improving the visceral sensation and diarrhea with SGJP in IBS-D rats might be realized through regulating NGF and its receptors.
出处 《中国中西医结合消化杂志》 CAS 2017年第4期253-257,共5页 Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金 国家自然科学基金(No:81302949)
关键词 腹泻型肠易激综合征 疏肝健脾方 神经生长因子. diarrhea-predominant irritable bowel syndrome Shuganjianpi prescription never growth factor
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