不同滴度噬菌体D29对气道上皮细胞9HTE的生长及功能影响研究

Effects of different titers of bacteriophage D29 on growth and function of airway epithelia cell 9HTE

目的研究噬菌体D29不同滴度对气道上皮细胞株9HTE的生长及功能影响。方法分别用高滴度(109PFU/mL)和低滴度(107 PFU/mL)噬菌体D29及噬菌体缓冲液组作用于9HTE细胞株后,用四甲基偶氮唑蓝比色法(MTT)检测细胞生长情况;用酶联免疫吸附试验(ELISA)法测量细胞上清液白细胞介素(IL)-6、IL-8水平;用逆转录PCR(RT-PCR)检测细胞间黏附分子-1(ICAM-1)mRNA的表达,用流式细胞技术检测细胞凋亡率。结果高、低滴度噬菌体D29作用气道上皮细胞一段时间后,对细胞生长活性、细胞因子IL-6、IL-8和ICAM-1mRNA的表达,以及细胞凋亡率的影响与噬菌体缓冲液组比较差异无统计学意义(P>0.05)。结论高、低滴度噬菌体D29对体外培养的气道上皮细胞生长及功能无明显影响。

Objective To research the effects of different titers of bacteriophage D29 on growth and function of airway epithelial cell 9HTE.Methods Cell viability rates was analyzed after applying high（109 PFU/mL）and low（107 PFU/mL）titers of bacteriophage D29 and phage buffer respectively by MTT colorimetry.Additionally,the secretion levels of IL-6,IL-8in cell culture supernatant were detected by ELISA.RT-PCR was performed to detect the expression of ICAM-1mRNA.Cell apoptosis rate was analyzed by flow cytometry.Results There was no difference in cell growth,secretion levels of IL-6,IL-8,ICAM-1mRNA and cell apoptosis rate between cells treated with high and low titers of D29 and phage buffer（P〉0.05）.Conclusion Neither high nor low titer of bacteriophage D29 exerts effect on growth and function of airway epithelial cell 9HTE in vitro.