幽门螺杆菌相关性胃腺癌中IL-37的表达及意义

IL-37 level in HP positive patients with gastric adenocarcinoma

目的探讨白细胞介素37(IL-37)在幽门螺杆菌(HP)相关性胃腺癌[HP(+)胃腺癌]中的表达及意义。方法选取2014年1月至2015年12月解放军第117医院收治胃腺癌78例(HP+46例、HP-32例),同期门诊HP(+)胃溃疡及HP(-)正常患者各30例。采用酶联免疫吸附法(ELISA)定量检测各组血浆IL-37蛋白表达;Western blot检测HP(+)和HP(-)胃腺癌组癌组织内IL-37蛋白表达。逆转录(RT)-PCR检测HP(+)与HP(-)胃腺癌癌组织IL-37 mRNA表达。结果 (1)各组血浆IL-37蛋白表达情况:HP(-)正常组与HP(-)胃腺癌组相比,差异无统计学意义(P>0.01);HP(+)溃疡组高于HP(-)正常组和HP(-)胃腺癌组,差异均有统计学意义(P<0.01);HP(+)胃腺癌组高于HP(+)溃疡组、HP(-)正常组及HP(-)胃腺癌组,差异均有统计学意义(P<0.01)。(2)胃腺癌组癌组织内IL-37蛋白表达情况:HP(+)组高于HP(-)组,差异有统计学意义。(3)胃腺癌组癌组织内IL-37 mRNA表达情况:HP(+)组高于HP(-)组,差异有统计学意义(P<0.01)。结论 HP感染后血浆IL-37升高,HP(+)胃癌组织内IL-37蛋白及mRNA表达均升高;IL-37表达升高与HP(+)胃腺癌的发生密切相关。

Objective To investigate the expression of interleukin-37 in Helicobacter pylori（HP）positive patients with gastric adenocarcinoma.Methods 78 cases of patients with gastric adenocarcinoma recruited in our hospital from January 2014 to December 2015 were selected.Among them,seventy-eight inpatients with gastric adenocarcinoma [HP（＋）46 cases,HP（-）32 cases],and 30 outpatients with HP（＋）gastric ulcer,and 30 outpatients with HP（-）were studied.The plasma IL-37 levels of each group were determined by ELISA.Western blot was used to test IL-37 protein in cancer tissue of HP（＋）and HP（-）cases with gastric adenocarcinoma.And RT-PCR was used to test IL-37 mRNA in cancer tissue of HP（＋）and HP（-）cases with gastric adenocarcinoma tissue.Results ① Expression of plasma IL-37 protein in each group： there were no statistically significant difference between HP（-）group and HP（-）cases with gastric adenocarcinoma（P〉0.01）.It was increased signifcantly in the HP（＋）ulcer group compared with HP（-）group and HP（-）cases with gastric adenocarcinoma（P〈0.01）,and it was increased signifcantly in HP（＋）cases with gastric adenocarcinoma compared with other groups（P〈0.01）.② Expression of IL-37 protien in gastric adenocarcinoma tissue： it was increased signifcantly in HP（＋）cases with gastric adenocarcinoma compared with HP（-）cases with gastric adenocarcinoma（P〈0.01）.③ Expression of IL-37 mRNA level in gastric adenocarcinoma tissue： it was increased signifcantly in HP（＋）cases with gastric adenocarcinoma compared with HP（-）cases with gastric adenocarcinoma（P〈0.01）.Conclusion The plasma IL-37 level was increased when patients were infected by HP,and the expression of IL-37 protein and mRNA were increased significantly in gastric adenocarcinoma tissue of HP（＋）patient with gastric adenocarcinoma.IL-37 might involved in the occurrence and development of gastric adenocarcinoma in patients with HP.