高迁移率族蛋白B1、白细胞介素-6和转化生长因子-β1在重度子痫前期患者外周血中的表达及意义

Expressions and significance of high mobility group box B1,interleukin-6,and transforming growth factor-β_1 in peripheral blood of patients with severe preeclampsia

目的探讨热高迁移率族蛋白B1(HMGB1)、白细胞介素-6(IL-6)和转化生长因子-β1(TGF-β1)在重度子痫前期(s PE)外周血中的表达。方法采用ELISA方法检测57例重度PE、21例正常晚孕对照组血清中HMGB1、IL-6和TGF-β1的表达。结果与正常晚孕对照组孕妇相比,重度子痫前期组孕妇HMGB1、IL-6、TGF-β1明显升高,差异有统计学意义(P<0.05)。早发型重度子痫前期中HMGB1高于晚发型重度子痫前期,但差异无统计学意义(P>0.05);早发型重度子痫前期中IL-6明显高于晚发型重度子痫前期,TGF-β1明显低于晚发型重度子痫前期,差异有统计学意义(P<0.05)。相关分析表明HMGB1与IL-6成正比,与TGF-β1成反比。结论 HMGB1增加可能参与PE的发病机制,评估细胞因子能有效监测重度子痫前期。

Objective To explore the expressions of high mobility group box B1 （ HMGB1 ）, interleukin-6 （ IL-6）, and transforming growth factor-β1 （ TGF-β1 ） in peripheral blood of patients with severe preeclampsia. Methods ELISA was used to detect the ekpressions of HMGB1, IL-6, and TGF-β1 in serum of 57 patients with severe preeclampsia and 21 normal late pregnant women （control group） . Results Compared with control group, the expressions of HMGBI, IL-6, and TGF-β1 in severe preeclampsia group increased, there were statistically significant differences （P〈0. 05 ） . HMGB1 expression in patients with early-onset severe preeclampsia was higher than that in patients with late-onset severe preeclampsia, but there was no statistically significant difference （P〉0.05） . IL-6 expression in patients with early-onset severe preeclampsia was higher than that in patients with late-onset severe preeclampsia, TGF-β1 expression in patients with early-onset severe preeclampsia was lower than that in patients with late-onset severe preeclampsia, there were statistically significant differences （P〈0. 05 ） . Correlation analysis showed that HMGB1 was positively correlated with IL-6, while HMGB1 was negatively correlated with TGF-β1. Conclusion HMGB1 upregulation may be involved in the pathogenesis of preeclampsia, assessment of cytokines can effectively monitor severe preeclampsia.