艾可清复方对艾滋病模型猴不同组织病毒载量及T细胞亚群的影响

Effect of Aikeqing Compounds(艾可清复方) on Viral Loads and T-lymphocyte Subsets in Different Tissue in Acquired Immune Deficiency Syndrome Model Monkeys

目的评价艾可清复方对艾滋病模型猴的干预效果。方法 8只同期猴免疫缺陷病毒(SIV)感染的恒河猴随机分为中药组和抗病毒药组,每组4只。中药组给予艾可清复方浸膏1 g/(kg·d)灌胃,抗病毒药组给予替诺福韦30 mg/(kg·d)背部皮下注射。各组均每天1次,共给药8周。给药前后留取外周血、浅表淋巴结以及实验结束时胃、胸腺、脾脏等不同组织,检测各样本中CD3、CD4、CD8水平,病毒载量和趋化因子受体CCR5水平,并进行病理观察。结果中药组外周血CD4水平及胃、肠系膜淋巴结、直肠中趋化因子受体CCR5表达高于抗病毒药组(P<0.05),且对胸腺、脾脏、浅表淋巴结组织病理结构保存更完整。抗病毒药组肠系膜淋巴结中病毒载量低于中药组(P<0.05)。结论艾可清复方对艾滋病模型猴有一定免疫调节作用。

Objective To evaluate the intervening effect of Aikeqing Compounds on acquired immune deficiency syndrome（AIDS） model monkeys. Methods Eight rhesus monkeys infected with Simian immunodeficiency virus（SIV） at the same time were randomly divided into the Chinese medicine group and antiviral agent group,with 4 monkeys in each group. The Chinese medicine group was given 1 g/（kg·d） of Aikeqing Compounds extract for gavage. The antiviral agent group was given 30 mg/（kg·d） of tenofovir for subcutaneous injection in back. Both groups were treated once daily for 8 weeks. Samples of peripheral blood and superficial lymph node were collected before and after medication,and tissue samples of s stomach,thymus and spleen were taken when experiment ended. Levels of CD3,CD4,CD8,chemokine receptor CCR5,and viral loads in different samples were examined. And then pathological analysis was conducted. Results Level of CD4 in peripheral blood,stomach and mesenteric lymph nodes,the expression of chemotactic factor CCR5 in rectum in the Chinese medicine group were higher than those in the antiviral agent group（P〈0. 05）,and pathologic structure of thymus,spleen and superficial lymph node tissue were conserved more complete in the Chinese medicine group. Viral loads in mesenteric lymph nodes in the antiviral agent group were lower than that in the Chinese medicine group（P〈0. 05）. Conclusion Aikeqing Compounds seems to have certain immunomodulatory effect on AIDS model monkeys.