阳江红树林内生真菌Aspergillus sp.5Y次级代谢产物及其活性的研究

The secondary metabolites and bioactivities of mangrove endophytic fungus Aspergillus sp. 5Y in Yangjiang

对阳江红树林内生真菌Aspergillus sp.5Y的次级代谢产物进行研究。本文采用硅胶柱层析、LH-20凝胶柱层析、ODS-C_(18)反相硅胶柱层析及HPLC等色谱技术,应用红外、紫外、质谱、核磁等现代波谱技术手段。通过对阳江红树林内生真菌Aspergillus sp.5Y进行次级代谢产物的研究,获得5个单体化合物,分别为6-epiophiobolin P(1)、strobilactone A(2)、penicisochroman E(3)、asperterpenol B(4)和(E,E)-6-(6',7'-dihydroxy-2',4'-octadienoyl)-strobilactone A(5)。在α-葡萄糖苷酶抑制实验中,化合物1,4对α-葡萄糖苷酶有显著的活性,IC_(50)分别为(676.09±10.3)μmol/L和(9.57±0.2)μmol/L,为口服降糖药物的研发提供一定的理论数据。

The secondary metabolites of mangrove endophytic fungus Aspergillus sp. 5Y was studied in this work. The chemical constituents were isolated and purified through silical gel, Sephadex LH -20, ODS- ClsCOlumnchromatography and HPLC and identified through IR, UV, mass anylysis and NMR specctroscopy. Five compounds were isolated from secondary metabolites of mangrove endophytic fungus Aspergillus sp. 5Y and their structures were identified as 6 - epi - ophiobolin P （ 1 ）, strobilactone A （2）, penicisochroman E （3）, asperterpenol B （4）, and （ E, E） - 6 - （6＇, 7＇ - dihydroxy - 2＇, 4＇-octadienoyl） -strobilactone A （5）. Compound 1, 4 have a good inhibition on ot -glucosidase, ICs0 values of （600. 13 ± 10.3） μmol/L, （9.57 ±0. 2） μmol/L respectively, which provides a certain theoretical data for researching and developing oral hypoglycemic drugs.