白细胞介素-13RS20541位点基因多态性与肠道病毒71型感染手足口病相关性

Association of interleukin - 13RS20541 gene polymorphism with enterovirus 71 infection in children with hand -foot -mouth disease

目的探讨白细胞介素－13RS20541（IL－13RS20541）位点基因多态性与肠道病毒71型（EV71）感染手足口病的关系。 方法以湘潭市中心医院123例确诊为EV71感染患儿为试验组，并根据其病情轻重分为轻症EV71感染组（轻症组，62例），重症EV71感染组（重症组，61例）。按年龄和性别匹配同期健康体检儿童52例为对照（健康对照组）。留取静脉血2 mL，－80 ℃冰箱冻存。送北京优博兰基因公司进行DNA提取及使用SNaPshot技术进行IL－13基因型检测。采用SPSS 18.0软件进行数据处理和分析。结果IL－13RS20541位点存在AA、GA、GG 3种基因型；试验组AA、GA、GG频率分别为4.07%、44.71%、51.22%，与健康对照组（AA、GA、GG频率分别为11.54%、32.69%和55.77%）比较差异无统计学意义（χ^2＝4.676，P〉0.05）；试验组等位A、G频率为26.42%、73.58%，与健康对照组（27.88%、72.12%）比较差异无统计学意义（χ^2＝0.080，P〉0.05）。EV71感染所致轻症组、重症组和健康对照组3组间基因型频率[（AA＋GA）分别为53.22%、44.26%、44.23%；GG分别为46.78%、55.74%、55.77%]及等位基因频率（A频率：30.65%、22.13%、27.88%；G频率：69.35%、77.87%、72.12%）比较差异均无统计学意义（χ^2＝1.294、2.349，均P〉0.05）。结论IL－13RS20541位点基因多态性与EV71易感性及EV71致病严重程度之间无明显相关性。

Objective To explore the relationship between interleukin（IL）-13 RS20541 gene polymorphism and susceptibility of enterovirus 71 （EV71） infection in children′s hand-foot-mouth disease.Methods Blood samples were collected from 123 children with EV71 infection from the Central Hospital of Xiangtan （experimental group）, and they were divided into mild EV71 infection group （n=62） and severe EV71 infection group （n=61） according to their severity.And 52 healthy children without EV71 infection were selected as the controls, with age and sex matched.Two mL blood samples stored in the -80 ℃ freezer, were collected from all subjects, and DNA was extracted by Beijing ubiolab genetic technology company limited.The SNaPshot was used to determine genotype for G/A polymorphism at RS20541 position of IL-13 gene.SPSS 18.0 software was used to analyze the data.Results IL-13 RS20541 loci had 3 genotypes： AA, GA, GG; the frequency of AA, GA, GG in the experimental group was 4.07%, 44.71%, 51.22%, which was significantly lower than that in the healthy control group （the frequency of AA, GA, GG were 11.54%, 32.69%, 55.77%）, there was no statistically significant difference in genotypes （χ^2=4.676, P〉0.05）; there was no statistically significant difference in allele frequency （the frequency of A, G in experimental group was 26.42%, 73.58%, and that of the healthy control group was 27.88%, 72.12%; χ^2=0.080, P〉0.05）. EV71 infection caused by mild group, severe group and healthy controls genotype frequencies between the 3 groups[（AA＋ GA）were 53.22%, 44.26%, 44.23%; GG were 46.78%, 55.74%, 55.77%; χ^2=1.294, P〉0.05] and allele frequency（A were 30.65%, 22.13%, 27.88%; G were 69.35%, 77.87%, 72.12%; χ^2=2.349, P〉0.05）among the mild group, severe group and healthy control group had no statistical significance.Conclusion There is no correlation between the IL-13RS20541 gene polymorphism and EV 71 infection in children with the hand-foot-mouth disease.