PTEN和EMMPRIN在肺腺癌组织中的表达及其与EGFR基因突变的关系

Expression of PTEN and EMMPRIN in Lung Adenocarcinoma Tissues and Their Correlation with EGFR Mutation

目的观察肺腺癌中PTEN和EMMPRIN蛋白水平的表达,分析其与肺腺癌临床病理特征及EGFR基因突变的关系。方法选取10例正常肺组织作为对照组,分别用免疫组织化学法和PCR法检测90例肺腺癌组织中PTEN和EMMPRIN蛋白的表达和EGFR基因突变情况。结果肺腺癌组织中PTEN蛋白的表达显著低于正常肺组织,EMMPRIN蛋白的表达显著高于正常肺组织。PTEN和EMMPRIN表达率为40%(36/90)和80%(72/90),EGFR基因突变率为47.78%(43/90)。PTEN的表达与肺腺癌组织学类型有关(P=0.000),EMMPRIN的表达与淋巴结转移状况及TNM分期有关(均P<0.05)。Spearman相关分析显示PTEN和EMMPRIN呈显著负相关(rp=-0.215,P=0.041),PTEN与EGFR基因突变呈正相关(rp=0.490,P=0.000),EMMPRIN与EGFR基因突变无明显相关性(rp=0.170,P=0.110)。结论肺腺癌中PTEN和EMMPRIN蛋白可能存在某些机制共同作用促进了肺腺癌的发生发展,PTEN蛋白的表达与EGFR基因突变呈正相关。

Objective To investigate the expression of PTEN and EMMPRIN and their relationships with clinicopathological factors and EGFR mutation in lung adenocarcinoma tissues. Methods The expression of PTEN and EMMPRIN proteins were examined in 90 cases of lung adenocarcinoma tissues by immuno- histochemical method, and 10 cases of normal lung tissues were taken as control. The mutation of EGFR was detected using PCR. Results PTEN expression in lung adenocarcinoma tissues was significantly lower than that in non-neoplastic internal controls tissues. EMMPRIN expression in lung adenocarcinoma tissues was significantly higher than that in internal controls tissues. The positive rates of PTEN and EMMPR1N expres- sion were 40%（36/90） and 80%（72/90）, respectively. EGFR gene mutation rate was 47.78%（43/90）. The ex- pression of PTEN was positively correlated with the histological subtypes（P=0.000）~ EMMPRIN expression was positively correlated with lymph node metastasis status and TNM stage（both P=0.000）. Spearman correla- tion analysis showed that PTEN expression was negatively correlated with EMMPRIN expression（rp=-0.215, P=0.041）. PTEN expression had a positive correlation with EGFR mutation（rp=0.490, P=0.000）. EMMPRIN expression had no correlation with EGFR mutation（rp=0.170, P=0.110）. Conclusion PTEN and EMMPRIN may have a synergistic effect on the progression and development of lung adenocarcinoma. PTEN expression has a positive correlation with EGFR mutation which may provide evidences for targeted therapy.