摘要
目的 运用体积排阻高效液相色谱-电感耦合等离子体质谱(SEC-HPLC-ICP-MS)监测碲化镉量子点(CdTe QDs)中元素出峰时间及峰面积,评价CdTe QDs在体内和体外的生物稳定性.方法 通过透射电镜和紫外荧光表征合成的水溶性CdTe QDs,将CdTe QDs分别加入二次水、流动相、牛血清基质中监测放置不同时间后稳定性变化;将CdTe QDs注入小鼠静脉中,分别于1、24、72 h后测定小鼠血清和肝脏中CdTe QDs形态的变化.采用体积排阻高效液相色谱(SEC-HPLC)将溶液中的化合物按照体积大小进行洗脱,洗脱液在线进入电感耦合等离子体质谱(ICP-MS)测定.通过114Cd和130Te同时流出的时间和摩尔比值对CdTe QDs进行定性,通过峰面积判断CdTe QDs是否有降解.结果 CdTeQDs用二次水稀释至浓度为0.5 mmol/L时室温避光放置60 min完全降解;用流动相稀释CdTe QDs为0.005 mmol/L未检测到CdTe QDs峰;在体外牛血清基质中CdTe QDs浓度为0.005 mmol/L可室温避光放置48 h,114Cd的峰面积为6 179 841~7 346 084,130Te的峰面积为1 077 913~1 191 066.CdTe QDs在染毒小鼠血清1h峰面积最高,114Cd和130Te分别为18 183 894、25 187 987;在染毒小鼠肝脏中CdTe QDs降解迅速,在染毒1h小鼠的肝组织匀浆中可观察到含Cd的CdTe QDs降解产物出现,24 h时CdTe QDs大部分降解.结论 该方法可用于CdTe QDs的生物稳定性的研究,且CdTe QDs进入生物体后在肝脏降解产生Cd2+,可能导致机体毒性反应.
Objective To investigate the peak time and peak area of elements in cadmium telluride quantum dots (CdTe QDs) using size exclusion chromatography-high-performance liquid chromatographyinductively coupled plasma mass spectrometry,as well as the biological stability of CdTe QDs in vivo and in vitro.Methods Transmission electron microscope and ultraviolet fluorescence were used for characterization and synthesis of water-soluble CdTe QDs,and CdTe QDs were added to double-distilled water,mobile phase,or bovine serum medium to observe the change in stability after different periods of time.CdTe QDs were injected into the vein of mice,and the changes in the morphology of CdTe QDs in serum and the liver were measured at 1,24,and 72 hours after exposure.Size exclusion chromatography-high-performance liquid chromatography was used for the elution of the compounds in the solution based on their volume,and then inductively coupled plasma mass spectrometry was performed for the eluent.The flow time of 114Cd and 130Te and molar ratio were used for qualitative analysis of CdTe QDs,and the peak area was used to judge whether CdTe QDs were degraded.Results CdTe QDs were diluted to a concentration of 0.5 mmol/L with double-distilled water and then placed in a dark place at room temperature;CdTe QDs were completely degraded after 60 minutes.CdTe QDs were diluted to a concentration of 0.005 mmol/L with a mobile phase,and the peak of CdTe QDs was not detected.After CdTe QDs were placed in a dark place at room temperature for 48 hours at a concentration of 0.005 mmol/L in bovine serum mediumin vitro,the peak area of 114Cd was 6179841-7346084,and the peak area of 13Te was 1077913-1191066.CdTe QDs had the highest peak area at 1 hour after exposure,and the peak areas of 114Cd and 13Te were 18183894 and 25187987,respectively.CdTe QDs were quickly degraded in the liver;at 1 hour after exposure,the degradation products of CdTe QDs containing Cd were observed in liver tissue homogenate,and CdTe QDs were largely degradedat 24 hours.Conclusion This method can be used to investigate the biological stability of CdTe QDs.CdTe QDs are degraded in the liver and produce Cd2+,which may cause toxic reaction.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2017年第3期217-220,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
基金项目:国家自然科学基金项目(81273131、81573201)
北京市自然科学基金项目、北京市教育委员会科技发展计划重点项目(KZ201510025027)
学科师资队伍建设一优秀学术带头人及团队交流培养项目
关键词
镉
量子点
色谱法
高压液相
电感耦合等离子体质谱
Cadmium
Quantum dots
Chromatography,high pressure liquid
Inductively coupled plasma mass spectrometry