自拟解郁方对抑郁症大鼠下丘脑-垂体-肾上腺轴相关激素的影响

The effects of antidepressant decoction drafted by ourselves on the hormones of hypothalamic pituitary adrenal axis in the depression rats

目的探讨自拟解郁方对慢性不可预见性温和应激(chronic unpredictable mild stress,CUMS)致抑郁症大鼠下丘脑-垂体-肾上腺(hypothalamic-pituitary-adrenal,HPA)轴相关激素的影响。方法 2014年12月—2015年3月,选择40只大鼠随机分为对照组、模型组、氟西汀组、解郁方组各10只。模型组、氟西汀组、解郁方组采用CUMS构建抑郁模型,对照组及模型组给予生理盐水灌胃,氟西汀组给予氟西汀灌胃,解郁方组给予自拟解郁方灌胃,持续21 d。用药结束后,进行敞箱实验、糖水偏好实验;检测大鼠血清促肾上腺皮质激素释放激素(corticotropin releasing hormone,CRH)、促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)、皮质酮(corticosterone,CORT)的含量。计量资料采用方差分析,两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果模型组水平穿越格数、竖立次数和糖水偏好水平分别为(44.18±2.60)个、(13.24±2.05)次、(0.549±0.019),明显降低对照组的(91.89±2.37)个、(23.46±2.13)次、(0.735±0.021),差异均有统计学意义(均P<0.05)。模型组CRH、ACTH、CORT水平分别为(72.62±7.98)pg/ml、(54.34±3.97)、(36.26±6.96)ng/ml,均高于对照组的(51.15±5.67)pg/ml、(40.27±3.16)、(23.75±6.34)ng/ml,差异均有统计学意义(均P<0.05)。与模型组相比,氟西汀组、解郁方组水平穿越格数、竖立次数和糖水偏好[(73.09±2.84)个、(19.26±2.14)次、(0.656±0.021)、(72.29±1.59)个、(19.19±2.17)次、(0.661±0.017)]明显增高,差异均有统计学意义(均P<0.05)。氟西汀组和解郁方组CRH、ACTH、CORT水平分别为(62.37±6.17)pg/ml、(45.61±3.25)、(25.61±5.35)ng/ml、(61.89±6.03)pg/ml、(45.31±3.34)、(25.37±5.26)ng/ml,均低于模型组,差异均有统计学意义(均P<0.05)。结论自拟解郁方对CUMS诱导的抑郁症大鼠HPA轴相关激素具有调节作用。

Objective To investigate the effects of antidepressant decoction drafted by ourselves on the hormones of hypothalamic pituitary adrenal axis in the depression rats induced by chronic unpredictable mild stress （CUMS）. Method From December 2014 to March 2015, 40 rats were randomly divided into four groups： control group, model group, fluoxetine group and antidepressant decoction group （n=10）. The depression rats of model group, fluoxetine group, antidepressant decoction group were build by CUMS, rats in control group and model group were given physiological saline by intragastric administration, rats in fluoxetine group were given fluoxetine by intragastric administration, rats in antidepressant decoction group were treated with antidepressant decoction drafted by ourselves by intragastric administration, for 21 days： After intragastric administration, the tests of open field and sucrose preference were operating; the contents of serum corticotropin releasing hormone （CRH）, adrenocorticotropic hormone （ACTH） and corticosterone （CORT） of rats were detected. The differences of measurement data between and in group were analyzed by ANOV or with LSD-t test, P〈0.05 was considered statistically significant. Results The scores in horizontal motion, vertical motion, and sugar preferences in the model group [ （44.18 ± 2.60）, （13.24 ± 2.05）, （0.549 ± 0.019） ]were lower than that of the control group[ （ 91.89 ± 2.37 ）, （ 23.46 -± 2.13 ）, （0.735 ± 0.021 ） ], the differences were statistically significant （all P〈0.05）. The. contents of serum CRH, ACTH, CORT levels in model group[ （72.62±7.98） pg/ml, （54.34± 3.97）, （36.26±6.96） ng/ml]were higher than that of control group [ （51.15 ±5.67） pg/ml, （40.27± 3.16）, （23.75 ± 6.34） ng/ml], the differences were statistically significant （allP〈0.05）. Comparing with the model group, the scores in horizontal motion and vertical motion, and sugar preferences of fluoxetine group [（73.09 ± 2.84） ,（19.26± 2.14）, （0.656 ± 0.021）] and antidepressant decoction group [ （72.29 ± 1.59）, （19.19 ± 2.17）, （0.661 ± 0.017） ] were significantly increased, the differences were statistically significant （all P〈 0.05）. The contents of serum CRH, ACTH, CORT levels in fluoxetine group [ （62.37± 6.17） pg/ml, （45.61 ± 3.25 ）,（25.61 ± 5.35 ） ng/ml]and antidepressant decoction group[（61.89 ± 6.03） pg/ml,（45.31 ± 3.34）,（ 25.37 ± 5.26） ng/ml]were lower than that of model group, the differences were statistically significant （all P〈0.05）. Conclusion The antidepressant decoction drafted by ourselves has good regulating effects on hormones of HPA axis in the depression rats induced by CUMS.