不同培养基对红心杉组培苗增殖及其生理的影响

Effects of Different Media on the Seedling Growth and Physiology in Cunninghamia lanceolata

以广西优良红心杉单株组培苗为试验材料,采用L9(33)正交试验设计研究不同基本培养基、TDZ、香蕉泥组合及其浓度对红心杉增殖及其生理的影响,并探讨其增殖生长与叶绿素、可溶性糖、游离脯氨酸含量及抗氧化酶活性间的关系,为优化红心杉无性系组培苗增殖培养条件提供参考。结果表明:基本培养基是影响红心杉组培苗增殖系数、叶绿素、可溶性糖含量的主要因子,TDZ是影响红心杉组培苗游离脯氨酸含量、SOD、POD、PPO活性的主要因子;红心杉组培苗的增殖系数随着基本培养基中大量元素浓度的增加呈上升趋势;随着基本培养基中大量元素、TDZ浓度的增加,叶绿素、可溶性糖、游离脯氨酸含量、抗氧化酶活性总体均呈现出下降趋势,适当添加香蕉泥有利于红心杉组培苗叶绿素、可溶性糖、游离脯氨酸的积累及POD、PPO活性的提高;MS+TDZ0.2mg·L^(-1)+香蕉泥100mg·L^(-1)是较适宜红心杉组培苗增殖生长的培养基,增殖系数与可溶性糖、游离脯氨酸含量、POD、CAT活性间呈正向相关,与叶绿素含量、PPO、SOD活性间呈显著正相关。

Effects of different basic media,thidiazuron （TDZ） concentration,amount of compounded banana mud added in the medium on the seedling growth and physiology of Cunninghamia lanceolata were studied by using L9 （34） orthogonal experimental design,with the excellent clones of C.lanceolata tissue culture seedlings.To investigate the relationship between seedling growth,the content of chlorophyll,soluble sugar,free proline content and enzyme activity were measured to provide a reference for the optimization of C.lanceolata multiplication culture.The results showed the basic medium was the main factor affecting the multiplication ratio,chlorophyll content and soluble sugar content,while the TDZ was the main factor affecting the proline content,SOD,POD,PPO activity of C.lanceolata seedlings.The multiplication ratio increased with the concentration of large elements in the basic medium.With the increase of large elements in the basic medium and TDZ concentration,the multiplication ratio and chlorophyll,soluble sugar,free proline content and antioxidant enzyme activity showed a trend of decrease.Appropriate addition of banana mud was conducive to the accumulation of chlorophyll,soluble sugar,free proline and the addition increased the POD and PPO activity.The best growth medium for the seedling growth of C.lanceolata was MS＋TDZ 0.2 mg·L-1＋banana mud 100 mg·L-1.There was positive correlations between the multiplication ratio and soluble sugar,free proline content,POD,CAT activity,and significant positive correlations between the multiplication ratio and the chlorophyll content,PPO,SOD activity.