Oct4与TGF-β信号通路相关因子在胃癌组织中的表达和相互关系

EXPRESSION AND CORRELATIONS OF OCT4 AND TGF-Β SIGNALING PATHWAY RELATED FACTORS IN GASTRIC CANCER

目的:探讨八聚体结合蛋白4(Oct4)与TGF-β信号通路相关因子在胃癌组织中的表达情况和相互关系。方法:57例胃癌患者手术切除的癌组织及对应正常胃组织,采用Western blotting法检测Oct4、TβRⅡ、Smad2、Smad3、E-Cadherin蛋白的表达情况,分析它们与胃癌临床病理特征的关系,以及胃癌组织Oct4表达与TβRⅡ、Smad2、Smad3、E-Cadherin的相关性。结果:胃癌组织Oct4蛋白的表达明显高于正常组织,TβRⅡ、Smad2、Smad3、E-Cadherin蛋白的表达明显低于正常组织(P<0.05)。胃癌组织Oct4、TβRⅡ、Smad2、Smad3、E-Cadherin表达与与肿瘤组织分型、临床分期、淋巴结转移和浆膜浸润有关(P<0.05)。胃癌组织Oct4表达与TβRⅡ、Smad2、Smad3、E-Cadherin呈负相关关系(r=-0.887、-0.912、-0.779、-0.879,P<0.01)。结论:胃癌组织Oct4高表达可参与胃癌的发生发展和浸润、转移,其机制可能与调节TGF-β信号通路,诱导细胞上皮间质转化有关。

Objective:To investigate the expression and correlations of Oct4 and TGF-β signaling pathway related factors in gastric cancer.Methods:Western blotting was used to detect the expression of Oct4,E-Cadherin and TGF-β signaling pathway related factors TβRⅡ,Smad2,Smad3 protein in 57 cases of gastric cancer and normal gastric tissue;as well as the relationships between above-mentioned factors and clinicopathologic feature of gastric cancer,and the correlations between Oct4 expression and TGF-β signaling pathway related factors were also analyzed.Results:Compared with normal gastric tissue,the Oct4 expression in gastric cancer increased obviously,while the TβRⅡ,Smad2,Smad3,E-Cadherin expression decreased obviously(P<0.05).In gastric cancer,the expression of Oct4,TβRⅡ,Smad2,Smad3,E-Cadherin were related to tumour tissue typing,clinical stage,lymph node metastasis and serosal invasion(P<0.05).In gastric cancer,the Oct4 expression was negatively related to TβRⅡ,Smad2,Smad3 and E-Cadherin(r=-0.887,-0.912,-0.779,-0.879,P<0.01).Conclusions:High expression of Oct4 in gastric cancer may be involved in the development,invasion and metastasis of gastric cancer.The mechanisms may be related to induced epithelial mesenchymal transition by regulating TGF-β signaling pathway.