不同温度对牛精子相关抗原11D基因的影响

Effect of Temperature on Bovine Sperm Associated Antigen 11D Gene

为探讨高温对精子膜蛋白牛精子相关抗原11D(sperm associated antigen 11,SPAG11D)表达水平的影响,试验采用了实时荧光定量PCR和Western boltting方法检测了牛睾丸原代细胞和娟姗牛、槟榔江牛、西门塔尔牛的正常精子及低活力精子中SPAG11D的表达。结果显示,牛睾丸原代细胞在不同温度(32、34、36、38和40℃)下培养24h,SPAG11D的mRNA及蛋白在36℃时表达水平最高,且高温时表达水平比低温时要高;培养48h时,SPAG11D的mRNA表达基本和24h相一致,但蛋白表达水平在高温时明显下降。此外,槟榔江牛和娟姗牛低活力精子中SPAG11D的蛋白表达量比正常精子明显降低,而在西门塔尔牛的精液中变化不明显。

To explore the influence of heat stress on the expression level of bovine sperm associated antigen 11D（SPAG11D）,which was sperm membrane protein,the mRNA and protein expression levels of SPAG11 D were tested in primary bovine testicular cells and sperm.The normal sperm and low activity sperm were collected from Jersey cattle,Bos bubalus and Simmental cattle.The results showed that mRNA and protein expression levels of SPAG11 D were highest at36 ℃than others（32,34,38 and 40 ℃）after primary bovine testicular cells cultured for 24 h.And the mRNA and protein expression levels were higher in high temperature groups than low temperature groups.The mRNA expression of SPAG11 Din 48hwas consistent with 24 h,but the protein expression levels decreased at high temperature（38and 40 ℃）.In addition,the protein expression of SPAG11 Dwas lower in low activity sperm than in normal sperm（Jersey cattle and Bos bubalus）,and there was no significant change in Simmental cattle sperm.