两性霉素B脂质体抗烟曲霉的研究

The antifungal effect of liposomal Amphotericin B against Aspergillus fumigatus

目的通过对两性霉素B脂质体(L-AmB)的烟曲霉抵抗实验的研究,初步探讨其抗真菌的机制,为临床的合理使用两性霉素B脂质体提供实验指导。方法将烟曲霉菌株复苏后,接种于沙保弱平板,恢复正常毒力。用洗液洗脱烟曲霉的孢子,进行两性霉素B脂质体及两性霉素B体外抗真菌的研究;用注射器吸取一定量的含烟曲霉孢子的溶液,注入小鼠的肺部,当确证其肺部感染了烟曲霉后,用L-AmB进行治疗,后期通过观察小鼠肺部的病理结果判断疗效并检测鼠肺部细胞在药物治疗后的细胞自噬相关基因LC3B,Beclin-1的水平。结果 L-AmB组的体内外抑菌作用强于AmB组且L-AmB可显著增加鼠肺部细胞的自噬水平。结论两性霉素B脂质体可通过显著的增加细胞的自噬水平,来有效的抵抗烟曲霉的感染。

Objective To explore the antifungal mechanism by Amphotericin B liposome （L-AraB） against Aspergillus fumigatus and provide guidance for the clinical rational use of L-AraB. Methods After being revived, the Aspergillus strain was inoculated and cultured on Sabouraudts agar plate. The elution of Aspergillus fumigates spores was injected into the mice＇s lungs. The infected mice were treated with L-AmB and Arab respectively. The efficacy of treatment was evaluated by observing the pathological changes of the lungs of mice and by detecting the levels of autophagy related genes LC3B and beclin-1. Results The in vivo and in vitro antifungal effect in L-AraB group was stronger than that in AmB group ; L-AmB significantly in- creased the level of autophagy in the lung cells of mice. Conclusion L-AraB can significantly increase the autophagy level of the cell to resist Aspergillus fumigatus infection effectively.