人非小细胞肺癌A549细胞异质性研究

Heterogeneity of human non-small cell lung cancer cell line A549

目的:分析肺癌A549细胞的异质性特征。方法:有限稀释法分离A549细胞的单个克隆细胞并扩大培养,对亲代与子代细胞进行生长、转移等特征的分析,了解A549细胞内部存在的异质性特征。结果:共获得6个A549细胞子细胞株,与亲代细胞比较,各子代细胞在细胞增殖能力和转移特征方面存在明显差异(P<0.05)。结论:A549细胞存在细胞增殖能力和转移特征的异质性,可能与肿瘤的发生转移相关。

Objective：To analyze the heterogeneity of lung cancer cell line A549.Methods： Limiting dilution was used to isolate the single clones of A549 cells and expanded for culture.Then, the growth and metastasis of the parental and offspring cells were analyzed to determine the heterogeneity of A549 cells.Results：Six sub-cell lines were isolated.There were significant differences in the proliferation and metastasis between the parental and offspring cells.Conclusion：A549 cells show heterogeneity of cell proliferation and metastasis, and the heterogeneity may be related to tumor metastasis.