下一代测序技术在临床病毒学检验中的应用

Next generation sequencing technologies in clinical virological detection

下一代测序（NGS）技术以其高效、快速的特点推动了DNA测序技术的飞速发展,并不断深化其在临床病毒学各领域的应用,包括未知病毒的检测、人类病毒组研究、病毒遗传进化分析、病毒耐药突变监测以及病毒与宿主相互作用研究等.但是由于NGS生物信息数据处理的复杂性以及实验室质量管理标准的缺乏等原因,对于NGS在我国临床实验室的开展应用与质量管控仍需积极探索.

Next generation sequencing technologies,providing high speed and throughput that contribute greatly to DNA sequencing,have many applications in clinical virology,including identification of novel or unsuspected viruses,evaluation of human viromes,investigation of viral genetic diversity and evolution,monitoring of antiviral drug resistance,and analysis of virus-host interaction.Meanwhile,due to the complexity in bioinformatics data processing and lack of quality management standards,further efforts and explorations need to be put in the application and quality management of NGS in clinical laboratory.