丰富环境对缺血再灌注性脑损伤大鼠神经功能修复的实验研究

Experimental Study of Enriched Environment on Neurological Functional Repair in Rats with Cerebral Ischemia-reperfusion Injury

目的:观察丰富环境对缺血再灌注性脑损伤后大鼠神经功能的影响。方法:将体质量(55±5)g的SD雄性大鼠36只采用简单随机化分组方式分为丰富环境组12只和标准环境组24只,分别运用丰富环境和标准环境干预30 d。采用Etho Vision XT 7.0动物轨迹分析系统监控大鼠的运动情况。30 d后采用改良的线栓法制作大鼠缺血再灌注脑损伤模型,对造模成功的大鼠放回各自的环境中继续干预。术后第7天采用7分评分方法对大鼠进行神经功能缺失评分,术后第14天采用Cat Walk步态分析系统评估缺血脑损伤后大鼠的步态变化。结果:丰富环境可以增加大鼠的自主运动水平,且缺血再灌注脑损伤后丰富环境组大鼠神经功能缺失评分降低,丰富环境组大鼠神经功能缺失评分与标准环境组比较差异有统计学意义(P<0.05);丰富环境组大鼠的步态明显好于标准环境组大鼠,且从量化的指标看,丰富环境组每只大鼠足部的压力、足部与接触面的接触面积明显较标准环境组好,差异有统计学意义(P<0.05)。结论:丰富环境可以改善大鼠自主运动,并且促进缺血再灌注脑损伤后大鼠的神经功能修复。

Objective ：To observe the effect of enriched environment on neurological function in rats with cerebral ischemia- reperfusion injury. Methods ： Sprague-Dawley rats （50-60 g） were randomly assigned to standard environment or enriched environ- ment for 30 days. Each rat＇s path in the different conditions was recorded using an automated tracking system. And the distance moved （m） ,velocity （m/s） ,mobile duration （s） and moving equency were analyzed by Etho Vision XT 7.0 of Noldus information technolo- gy company. After 30 days, ischemia was induced by occluding the middle cerebral artery for 90 min, followed by reperfusion, and the successful rats were put back into their environment. After 7 days of the operation, fimctional outcomes were assessed using neurologi- cal deficits scores. CatWalk was used to assess gait changes of rats on day 14 after surgery. Results ：The enriched environment rats were significantly more active than the standard environment rats. The neurological deficit score of the standard environment group was significantly higher than that of the enriched environment group （P〈0.05）. The functional outcomes of rats in the enriched envi- ronment group were better than those in the standard environment group. In the enriched environment group, the gait of rats was signif- icantly better than that of the standard environment group. There were significant statistical differences between the enriched environ- ment group and the standard environment group for the pressure of the foot, the area of foot contact with the contact surface of rats （P〈 0.05）. Conclusion：Enriched environment can promote the autonomic movement of rats, and the enriched environment can pro- mote the neurological function recovery of rats after cerebral ischemia/reperfusion injury. The CatWalk gait analysis system can sensi- tively and objectively evaluate the motor function of the rats after cerebral i sehemiaJreperfusion injury.