金匮肾气丸对肾阳虚证模型大鼠股骨皮质骨差异蛋白质表达的影响

Effect of Jingui Shenqi Pill on the Differential Proteins in Femoral Cortical Bone of Kidney-yang Deficiency Rats Model

目的:从差异蛋白质角度探讨金匮肾气丸治疗肾阳虚证的机理。方法:建立肾阳虚证大鼠模型,设立正常组、肾阳虚组和中药组(金匮肾气丸组),双向电泳技术展示股骨皮质骨蛋白质组图谱,比较3组间差异蛋白点并进行质谱分析及鉴定。结果:3组股骨皮质骨双向电泳图谱的蛋白点数目均为480个。与正常组比较,肾阳虚组有16个差异表达的蛋白质。经金匮肾气丸治疗后,肾阳虚模型大鼠股骨皮质骨中表达上调的载脂蛋白A-I和线粒体热休克蛋白60及表达下调的原肌球蛋白异构体2α-3链、GDP解离抑制因子β、I型胶原α-1链和LOC683667等6个蛋白均出现表达趋势的反向调整。结论:金匮肾气丸治疗肾阳虚证可能与调整大鼠股骨皮质骨中原肌球蛋白异构体2α-3链、GDP解离抑制因子β、I型胶原α-1链、LOC683667蛋白、载脂蛋白A-I和线粒体热休克蛋白60等6个相关蛋白的表达有关。

Objective：To study on the mechanism of Jingui Shenqi pill treatment of kidney-yang deficiency from the angle of dif- ferential protein. Methods： We established the model of kidney-yang deficiency rats, set up the normal group, the kidney-yang defi- ciency group and the traditional Chinese medicine group （Jingui Shenqi pill group）. We used two-dimensional electrophoresis （ 2-DE ） to show the proteomic maps of femoral cortical bone, compared the differential protein spots between the groups, analyzed and iden- tified them by mass spectrometry. Results：There were 480 discernible proteins points in each 2-DE gel map. Compared with the nor- mal group,the kidney-yang deficiency group had 16 differentially expressed proteins. After treatment with Jingui Shenqi pill,in the kidney-yang deficiency model rats, the up-regulated expression of Apoa 1, Hspdl and the down-regulated expression of Tpm3,Arhgdib, Collal and Sri were back-adjusted. Conclusion：Jingui Shenqi pill in the treatment of kidney-yang deficiency is related to adjust the protein expression of femoral cortical bone in rats. Those proteins involve Apoal, Hspdl, Tpm3, Arhgdib, Collal and Sri.