NAC-1在膀胱癌中的表达及其与血管生成的相关性研究

The Expression of NAC-1 in Bladder Cancer and the Correlation with the Angiogenesis

目的:检测NAC-1在膀胱癌及癌旁组织中的表达,及其与膀胱癌临床病理的相关性研究,并进一步探讨其与血管生成的关系,为阐明膀胱癌的发生发展机制提供基础。方法:采用免疫组化法检测88例膀胱癌和30例癌旁组织石蜡块中NAC-1的表达情况,用si-RNA干扰技术下调膀胱癌细胞系T-24中NAC-1的表达,然后通过q RT-PCR方法检测干扰组及对照组中促血管生成因子(VEGF、b FGF、EGFR)的表达。结果:膀胱癌组织和癌旁组织中NAC-1的阳性表达率分别为79.54%和13.33%,膀胱癌组织显著高于癌旁组织(P<0.01);NAC-1表达与膀胱癌的肿瘤级别、肌肉浸润程度及淋巴结转移显著相关(P<0.05),在高级别、肌肉浸润及淋巴结转移的膀胱癌中呈现高表达,细胞实验发现NAC-1下调能够抑制促血管生成因子(VEGF、b FGF、EGFR)的表达。结论:NAC-1作为肿瘤相关基因在膀胱癌中高表达,并与膀胱癌的临床病理密切相关,其能调节促血管生长因子,可能会在肿瘤的血管生成及转移中具有重要的作用。

Objective： To detect the expression of NAC-1 gene in human Bladder cancer and corresponding non-tumor tissues, and explore the correlation between the expression of NAC-1 gene and the bladder cancer clinical pathology and angiogenesis, With the purpose of providing a basis for elucidating the pathogenesis of bladder cancer. Methods： The expressions of NAC-1 in 88 cases of Bladder cancer and 30 cases of adjacent tissues were studied by using immunohistochemical method. And qRT-PCR was used to detect the expression of angiogenesis factor （VEGF, bFGF, EGFR） after Si-RNA interfered NAC-1 of T-24 bladder cancer cell line. Results： The positive expression rate of NAC-I in bladder cancer and adjacent tissues were 79.54 % and 13.33 % respectively （P〈0.01）; the expression of NAC -1 gene was significantly related to the TMN stage, myometrial invasion degree and lymphatic metastasis of bladder cancer （p〈0.05）; Cell experiments showed that down-regulation of NAC-I inhibited the expression of pro-angiogenic factors（VEGF, bFGF, EGFR）. Conclusions： NAC-1, as a tumor associated gene, is highly expressed in bladder cancer and is closely related to the clinical pathology of bladder cancer. Furthermore, NAC-1 can regulate pro-angiogenic growth factors, which plays an important role in the study of the proliferation and metastasis of bladder cancer.