高氧对雄激素敏感的前列腺癌细胞移植瘤生长及其缺氧诱导因子-1α表达的影响

Effects of Inner Hyperoxia on the Growth and HIF-1α Expression of Androgen Sensitive Prostatic Cancer Cell Transplantation Tumor

目的:探讨高氧对雄激素敏感的前列腺癌细胞移植瘤生长及其缺氧诱导因子-1α表达的影响。方法:将前列腺癌前列腺淋巴结癌(LNCa P)细胞接种于36只Foxn1小鼠的双侧腹,并将其随机放置于含氧量不同的气室中并分组如下:缺氧组11例,常氧组16例,高氧组9例。处理28天后进行称重,麻醉处死,从左心室取血样;分离出移植瘤并进行称重。采用Western blotting、免疫荧光分析、血红蛋白测定的方法对各组移植瘤生长、血管生成及血管化、缺氧诱导因子-1(HIF-1α)表达以及细胞信号转导因子表达进行检测。结果:缺氧组的移植瘤生长较常氧组快(P<0.05);高氧组移植瘤生长与常氧组相比差异不具有统计学意义(P>0.05)。高氧组移植瘤的HIF-1α、血管内皮生长因子(VEGF)和血管内皮生长因子受体2(VEGF-R2)表达均较常氧组高,而缺氧组移植瘤的HIF-1α表达与常氧组基本相似。缺氧组移植瘤的血[HB]增长率(175%)高于常氧组(45%)。高氧组的Nrf2的表达水平较常氧组明显增加(P<0.05)。结论:体内高氧诱导HIF-1α在LNCa P肿瘤高表达的同时,不会加快肿瘤的生长。

Objective： To investigate effects of inner hyperoxia on the growth and H1F-1 c~ expression of androgen sensitive prostatic cancer cell transplantation tumor. Methods： Prostate cancer LNCaP cells were inoculated in the bilateral abdomen of 36 Foxnl mice, which were placed randomly in the air chamber with disparate oxygen content, including hypoxia group with 11, normoxia group with 16 and hyperoxia group with 9. Mice were weighed and then killed by anaesthesia for blood in left ventricular after 28 days. The transplantation tumor was isolated and weighed as well. The transplantation tumor growth, angiogenesis and vascularization, HIF-1α expression and cell signal transduction factors of each group were tested by western blotting, immunofluorescence and hemoglobin detection. Results： The transplantation tumor ofhypoxia group developed more rapidly than the normoxia group （P〈0.05）. The showed no statistical difference was found in the growth of transplantation tumor between nomoroxia and hyperoxia group （P〉0.05）; the HIF-1α, VEGF and VEGF-R2 expressions of transplantation tumor in the hyperoxia group were higher than those of the normoxia group（P〈0.05）. However, the HIF-1α expression of transplantation tumor in hypoxia group was similar to that of the normoxia group. The hemoglobin growth rate （175%） of transplantation tumor in hypoxia group was higher than that of the normoxia group （45%）. The Nrf2 expression of hyperoxia group was overtly higher than that of the normoxia group （P〈0.05）. Conclusions： High expression level of HIF-1α was induced by inner hyperoxia, but it could not simultaneously accelerate the tumor growth.