百里香酚脂质体的制备及其性能的研究

Study on preparation and properties of thymol liposome

采用乙醇注入法和薄膜蒸发法制备百里香酚脂质体,考察制备方法及不同酚脂比对其平均粒径、多分散指数、包埋率、抗氧化能力、还原能力和抑菌能力的影响。结果显示,薄膜蒸发法所制得的脂质体的平均粒径与乙醇注入法制得的脂质体相当,但分散性、包埋率优于后者。在酚脂比为1.4mg/m L时,薄膜蒸发法制得的脂质体平均粒径为278.3nm,多分散指数为0.471,其形态为接近圆形,且其分散较为均一。酚脂比为1.8mg/m L时,薄膜蒸发法制得的脂质体的DPPH自由基清除能力为87.60%,对大肠杆菌的抑菌能力为56.60%,表明百里香酚经脂质体包埋后仍具有较好的抑菌和抗氧化性能。

Thymol hposomes were prepared by ethanol injection method and thin film evaporation method;the effects of preparation methods and different thymol/lipidratio on average particle size,poly dispersity index,entrapment efficiency,antioxidant capacity,reducing ability and antimicrobial effects were investigated.The results showed that the particle size of liposomes prepared by thin film evaporation method was comparable with that of ethanol injection method,but the dispersion and encapsulation efficiency were better than that of the latter.When thymol/lipidratio was1.4mg/mL,the average particle size of the liposome was 278.3nm,the dispersion index was 0.471,the shape was close to the spherical,and its dispersion was uniform.When liposome prepared by thin film evaporation method at mlthymol/lipid ratioof 1.8mg/mL,the DPPH free radical scavenging capacity was 87.60%,antibacterial ability against Escherichia coliwas 56.60%,indicating that thymol liposome had good antimicrobial and antioxidant properties.