非类固醇类抗炎药NS398对肺癌H460细胞RECK及MT1-MMP表达的影响

Effect of NS398 on expressions of reversion-inducing-cysteine-rich protein with kazal motifs and membranetype 1-matrix metalloproteinase in human H460 lung carcinoma cells

目的 探讨非类固醇类抗炎药 NS398对肺癌 H460细胞增殖及其 Kazal基序逆向诱导 半胱氨酸丰富蛋白 （RECK）及膜型基质金属蛋白酶1 （MT1-MMP）表达的影响。方法 应用 MTT 方法检测 H460细胞生长的抑制率,用免疫荧光法、Westernblot法检测 RECK 及 MT1-MMP蛋白的 表达。结果 NS398可抑制 H460细胞的增殖,促进 RECK 蛋白的表达,减少 MT1-MMP 蛋白的含 量,并呈剂量依赖关系。结论 NS398可通过促进肺癌细胞 RECK 的表达并减少 MT1-MMP的含量, 进而抑制肺癌 H460细胞的生长,这可能是肺癌侵袭转移的一个重要机制。

Objective To explore the effect of NS398 on the expressions of reversion-inducing- cysteine-rich protein with kazal motifs （RECK） and membrane type 1-matrix metalloproteinase （MT1 MMP） in human H460 lung carcinoma cells. Methods The H460 ceil growth inhibition rate was detected by MTT assay. Immunofluorescence assay and Western blot were used to analyse the expressions of RECK and MT1-MMP protein levels in H460 cells. Results NS398 inhibited the proliferation of H460 cells, promoted the expression of RECK protein, reduced the expression of MT1-MMP protein in a dose dependent manner. Conclusions NS398 may increase the expression of RECK and decrease the expression of MT1-MMP in lung carcinoma cells, which can inhibit the growth of H460 ceils. It is probably a key mechanism of NS398 regulating invasion and migration of lung carcinoma cells.