冷驯化下中缅树鼩脂肪组织PET/CT扫描成像研究

Studies of PET/CT Scan Imagines of Adipose Tissue in Tupaia belangeri under Cold Acclimation

为了研究中缅树鼩冷驯化条件下脂肪组织的产热活性及白色脂肪组织是否会褐变,实验首次利用正电子发射断层显像/X射线计算机体层成像仪(PET/CT)对中缅树鼩脂肪组织代谢活性进行了探讨,并通过不同的药物浓度梯度实验,首次成功获取中缅树鼩的最佳注射浓度为110~160μCi,此时能够获得最有效的示踪剂辐射值。实验结果表明:随着冷驯化时间的延长,冷驯化0 d、28 d、49 d中缅树鼩褐色脂肪组织的PET/CT扫描成像差异显著。冷驯化28 d时颈部、肩胛、腋下等褐色脂肪组织的平均SUV值与对照组相比差异显著,至49 d时分别增加了10.4倍、9.8倍、13.65倍。冷驯化28 d中缅树鼩腹股沟、腹部大网膜、肠系膜等白色脂肪组织的平均SUV值与对照组相比差异显著,至49 d时分别增加了5.5倍、2.56倍、5.17倍。结果说明,在冷驯化下,中缅树鼩通过增强褐色脂肪组织产热活性,以提高产热应对冷胁迫。冷驯化使中缅树鼩白色脂肪组织增加了18F-FDG的吸收值,意味着此时树鼩白色脂肪组织中可能褐变产生了米色脂肪细胞。

In order to study the thermogenic activity of adipose tissue and whether white adipose tissue induce'browning'under the cold acclimation in Tupaia belangeri. The first use of positron emission tomography-computed tomography( PET/CT) has carried on the discussion to the Tupaia belangeri metabolic activities of adipose tissue,and through the different drug concentration gradient experiments we first successfully acquire the best injection concentration 110 ~ 160 μCi in Tupaia belangeri,which can obtain the most effective tracer radiation values. The results showed that with cold acclimation time prolonged,PET/CT scan imaging in brown adipose tissue( BAT) on day 0,28,49 showed significant differences in Tupaia belangeri. The average SUV value of the neck,shoulder blade,armpit and other BAT were significantly different from that of the control group,and increased by 10. 4times,9. 8 times and 13. 65 times compared with the control group at 49 d,respectively. Inguinal,abdominal greater omentum,mesentery white adipose tissue( WAT) mean SUV values in 28 d of cold acclimation group of Tupaia belangeri had significant difference compared with that of control group,which increased by 5. 5 times,2. 56 times,5. 17 times compared with the control group at 49 d. All of the results showed that during the cold acclimation,Tupaia belangeri enhanced BAT thermogenic activity to improve the heat production,and it also showed that WAT increased 18F-FDG uptake values under cold acclimation in Tupaia belangeri,which means WAT may produce the beige fat cells.