不同核酸检测方法对献血者隐匿性乙肝病毒感染的分析

Analysis of Effect of Different Nucleic Acid Test Methods on the Occult Hepatitis B Virus Infection of Blood Donors

目的分析不同核酸检测方法对献血者隐匿性乙肝病毒感染的检测能力。方法方便抽取2015年1月—2016年10月期间到献血站进行无偿献血的8 000名人员,对其分别选择Procleix Ultrio以及nested-PCR两种自动核算检测系统对其血浆标本予以HBN核酸检测,同时序列分析核酸阳性标本。结果通过研究后可知,40名献血者HBs Ag检测结果为阳性,比例经计算后为0.50%,420名献血者抗-HBc结果为阳性,比例经计算后为5.25%,25例献血者经过Procleix Ultrio以及nested-PCR能力相当,表现为阳性结果。结论献血人员中隐匿性乙肝病毒感染比例较高,而核酸检测方法的差异性致使检测能力有所不同。

Objective To analyze the effect of different nucleic acid test methods on the occult hepatitis B virus infection of blood donors. Methods 8 000 cases of blood donors from January 2015 to October 2016 were extracted and the plasma specimens were given the HBN nucleic acid test respectively by the Procleix Ultrio and nested-PCR, at the same time, the nucleic acid positive samples were given the sequence analysis. Results The research showed that of the 40 blood donors,the HBs Ag test result was positive accounting for 0.50%, of 420 cases of blood donors, the anti-HBC result was positive,accounting for 5.25%, the result of Procleix Ultrio was similar to that of nested-PCR, positive. Conclusion The ratio of occult hepatitis B virus infection of blood donors is higher, and the difference in the nucleic acid test method lead to different test abilities.