法氏囊多肽BSP-Ⅰ和BPP-Ⅰ的免疫调节活性及其B细胞结合蛋白的鉴定

Immune regulation of BSP-I and BPP-I and their binding proteins with B cell

为了研究法氏囊多肽的免疫调节作用,本试验用BSP-Ⅰ和BPP-Ⅰ分别与禽流感灭活疫苗或抗原混匀后免疫鸡。结果显示,BSP-Ⅰ和BPP-Ⅰ均明显提高免疫鸡体内禽流感抗体水平,且两者均促进Th1和Th2型细胞因子产生。采用鸡B淋巴细胞cDNA T7噬菌体文库筛选法氏囊活性肽相互作用的受体蛋白,PCR扩增富集噬菌体克隆的插入片段。序列分析结果表明,BSP-Ⅰ相互作用的蛋白受体有3种,分别为VSTM2、N-钙黏蛋白和v-myc。BPP-Ⅰ相互作用的蛋白分子主要是Ryanodine受体、MGC83917蛋白和BTB/POZ1。这些结果表明,法氏囊活性肽参与机体中多种生物学过程,为深入研究法氏囊活性肽的功能提供了理论基础。

To evaluate the immune inducing activity of BSP-I and BPP-I in chickens, 75 day-old chickens were co-immunized with both bursal peptides and avian influenza virus （AIV） vaccine or antigen. The results showed that BSP-I and BPP-I were able to significantly in- duce the immunized chicken to produce the HI antibody against AIV, and stimulated Thl cytokine （IFN-γ） and Th2 cytokine （IL-4） pro- duction after AIV vaccine or antigen immunization. Then T7 cDNA from avian B cell phage display library was used to screen the interacting proteins with BSP-I and BPP-I in B lymphocyte. The homology sequence alignment results from these enriched phages showed that three kinds of proteins N-cadherin （neuronal）, V-set and transmembrane domain containing 2 and v-myc might interact with BSP-I. BPP-I might interact with cardiac muscle ryanodine receptor, MGC83917 protein and BTB/POZ1. These results suggested that the bursal-derived peptides BSP-I and BPP-I might participate in a variety of biological functions, which provides the basis for investigation of the role of bur- sal-derived peptides in immune induction.