人肝癌细胞HepG2接种于BALB/C裸小鼠移植瘤的性别差异

Gender difference of human hepatocellular carcinoma HepG2 cell xenografts in BALB/C nude mice

目的研究性别对人肝癌细胞Hep G2接种于BALB/C裸小鼠成瘤及移植瘤生长趋势的影响。方法 18只4~6周龄BALB/C裸小鼠雌雄各半,按照性别分成雌雄两组,每组9只,将全部裸小鼠肩胛部接种处于对数生长期且浓度为1.0×107/m L的Hep G2细胞0.2 m L,10 d后观察两组的成瘤情况并测量肿瘤体积。结果雌性组成瘤模型4只,成瘤率为44.44%;雄性组成瘤模型9只,成瘤率为100.00%,差异有统计学意义(P=0.029);造模成功的移植瘤体积走势图显示移植瘤生长速度雄性组大于雌性组。结论人肝癌细胞Hep G2接种于BALB/C裸小鼠成瘤率及移植瘤生长速度有明显的性别差异。

Objective To study the effect of gender on the tumorigenicity and growth trend after human hepatocellular carcinoma Hep G2 cells were inoculated into BALB/C nude mice. Methods Eighteen 4-6 weeks’ old BALB/C nude mice were divided into two groups（male group and female group） according to the gender, with 9 in each group.All the nude mice scapular were inoculated with 0.2 m L Hep G2 cells in logarithmic growth phase and at the concentration of 1×107/m L. After 10 days, the tumor formation of the two groups was observed, and the tumor volume was measured. Results The number of tumorigenicity model in female group was 4, and tumorigenicity rate was 44.44%. The number of tumorigenicity model in male group was 9, and tumorigenicity rate was 100.00%, with statistically significant difference（P=0.029）. The transplanted tumor volume trend figure of the successful model showed that the tumor growth in male group was higher than that in the female group. Conclusion Tumorigenicity rate and growth rate of transplanted tumor have obvious gender difference after human hepatocellular carcinoma Hep G2 cells were inoculated into BALB/C nude mice.