大豆质核互作雄性不育系NJCMS1A与其保持系NJCMS1B的生理生化特性比较分析

Comparative Analysis of Physiological and Biochemical Characteristics Between Cytoplasmic-Nuclear Male Sterile Line and Its Maintainer in Soybean [Glycine max( L.) Merr.]

以大豆质核互作雄性不育系NJCMS1A及其保持系NJCMS1B的花芽为试验材料,利用试剂盒法和紫外分光光度计测定分析了糖分含量(可溶性糖和淀粉)和总ATPase、蔗糖磷酸合成酶(SPS)、磷酸烯醇式丙酮酸羧激酶(PEPCK)、抗坏血酸氧化酶(AO)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)以及过氧化物酶(POD)等酶活性的变化情况。结果表明:相较于保持系NJCMS1B,总ATPase、PEPCK、AO和CAT活性在不育系NJCMS1A中显著下降;糖分含量、SPS和SOD活性在不育系NJCMS1A中下降水平不显著;POD活性在不育系NJCMS1A中显著上升。根据结果分析推测,与能量代谢或胁迫响应等相关的物质或酶活性在不育系NJCMS1A中的亏损现象可能是NJCMS1A花粉败育的主要原因。

With the young flower buds of the cytoplasmic-nuclear male sterile line NJCMS1 A and its maintainer NJCMS1 B of soybean as test materials, the content of sugar （ soluble sugar and starch）, the activities of total ATPase, sucrose phosphate synthase （SPS）, phosphoenolpyruvate carboxykinase （PEPCK）, ascorbate oxidase （AO）, catalase （CAT）, superoxide dis- mutase （SOD） and peroxidase （POD） were measured using the kit method and UV-spectrophotometer. The results showed that the total ATPase, PEPCK, AO and CAT activity were significantly decreased and total sugar content, SPS, and POD ac- tivity non-remarkably decreased in the male sterile line NJCMS1A, compared with its maintainer NJCMSI B; the activity of POD showed a significant increase in the male sterile line NJCMS1A, compared with its maintainer NJCMS1 B. According to the present analysis results, we concluded that the deficiency phenomenon of substances or enzymes activity associated with energy metabolism or stress response in male sterile line NJCMS1A of soybean, may be the main reason for poller abortion.