人白细胞介素-33在肾细胞癌中高表达并通过EMT促进肾癌细胞的侵袭能力

High expression of interleukin-33 in renal cell carcinoma which promoting cell invasion by EMT

目的:研究人白细胞介素(interleukin,IL)-33在肾细胞癌中的表达情况及在肾癌细胞786-0侵袭中的作用。方法:应用免疫组化法检测34例不同级别肾脏细胞癌患者肾脏肿瘤组织及癌旁组织中IL-33表达水平;应用细胞侵袭实验检测IL-33对肾癌细胞786-0侵袭的影响;应用Western blot检测IL-33对肾癌细胞786-0上皮细胞间质转化(epithelial-mesenchymal transition,EMT)相关蛋白表达的影响。结果:免疫组化结果显示,与癌旁组织相比,肾细胞癌患者肿瘤组织中IL-33水平明显升高,半定量统计分析结果表明两者间存在统计学差异(P<0.001)。体外实验表明,IL-33可促进786-0细胞侵袭;Western blot结果显示,用IL-33刺激786-0细胞后细胞内E-cadherin蛋白表达量明显降低,而vimentin蛋白表达量明显升高。结论:IL-33在肾细胞癌组织中表达明显升高,并可能通过诱导肾癌细胞发生EMT进而提高肾癌细胞的侵袭能力。

Objective：To detect the expression of interleukin（IL）-33 in renal cell carcinoma（RCC） and its effect on invasion of human 786-0 cells.Methods：IL-33 protein in 34 patients with different grades of RCC tissues and para-adjacent tissues was detected with immunohistochemistry assay.Transwell invasion assay was used to investigate the effect of IL-33 on invasion of 786-0 cells.Western blot assay was conducted to detect the markers of epithelial-mesenchymal transition（EMT） in 786-0 cells after IL-33 treatment.Results：IL-33 protein expression was significantly higher in RCC tissues compared to para-adjacent renal tissues（P〈0.001）.IL-33 enhanced 786-0 cells invasion in vitro.Moreover,the expression of E-cadherin was significantly decreased while the expression of vimentin was significantly increased after IL-33 treatment.Conclusion：IL-33 is overexpressed in RCC tissues and may contribute to renal cancer cells invasion by inducing EMT.