川崎病合并冠脉损伤患儿血清抑制HUVECs增殖并诱导其凋亡

Serum from children with coronary artery lesions of Kawasaki disease inhibits HUVECs proliferation and induces the apoptosis

目的:观察川崎病(Kawasaki disease,KD)合并冠脉损伤患儿血清对脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)增殖及凋亡的影响,以进一步了解KD患儿血管内皮细胞损伤的机制。方法:HUVECs分为正常血清组(Control组)、发热血清组(F组)、KD无冠脉损伤组(Non-CALs组)和KD合并冠脉损伤组(CALs组)。MTT比色法检测细胞增殖,流式细胞术(flow cytometry,FCM)分析各组HUVECs的凋亡情况。结果:MTT比色结果显示,Non-CALs组和CALs组经血清干预后的细胞活性低于F组和Control组,且CALs组低于Non-CALs组,差异有统计学意义(P<0.01)。此外经KD患儿血清作用后,HUVECs的凋亡率增加,与对照组相比差异有统计学意义(P<0.01),且CALs组凋亡率高于Non-CALs组。结论:KD合并冠脉损伤患儿血清具有抑制HUVECs增殖并诱导HUVECs凋亡的作用。

Objective： To observe the effects on proliferation and apoptosis in human umbilieal vein endothelial cells（HUVECs）cultured with the serum of the children with coronary artery lesions of Kawasaki disease, in order to further understand the possible mechanism of vascular endothelial injury from Kawasaki disease. Methods： HUVECs were cultured and divided into 4 groups, including the normal serum group, the general fever group, the non-CALs group, and the CALs group. The proliferation was detected by MTT test and the apoptosis was detected by flow cytometry（FCM）. Results： The OD value of the HUVECs detected by MTT test after being cultured with the serum of the children with Kawasaki disease including the Non-CALs group and the CALs group（0.492±0.033 and 0.395±0.031, respectively） were lower than that of the general fever group and the normal serum group（0.592±0.033 and0.612±0.036, respectively）（P〈0.01）. The apoptosis of HUVECs detected by FCM after being cultured with the serum of the children with Kawasaki disease was higher than that of the other two groups（P〈0.01）, and the apoptosis of CALs group was higher than that of the Non-CALs group. Conclusion： The serum from children with coronary artery lesions of Kawasaki disease could inhibit the proliferation of HUVECs and induce the apoptosis.