何首乌饮对衰老大鼠生精细胞线粒体凋亡通路关键基因表达的影响

Effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats' spermatogenic cells

目的探讨何首乌饮对衰老大鼠睾丸生精细胞线粒体凋亡通路关键基因表达的影响。方法选用12月龄Wistar雄鼠45只,随机分为3组:青年对照组、自然衰老组、何首乌饮组,每组15只,何首乌饮组和自然衰老组于16个月开始分别灌胃何首乌饮和等量蒸馏水,连续60d。青年对照组于12月龄,其余两组于18月龄分别进行实验,通过Real-time PCR、免疫荧光染色和免疫印迹法,观察各组大鼠睾丸组织线粒体凋亡通路关键基因DR6、BAX、Caspase-3、Cyt-C、14-3-3σ的表达变化。结果自然衰老大鼠线粒体凋亡通路关键基因14-3-3σ表达明显低于青年对照组,DR6、BAX、Caspase-3、Cyt-C表达明显高于青年对照组;而何首乌饮用药后能明显逆转上述现象。结论何首乌饮提高衰老大鼠精子质量与线粒体凋亡通路有关。

Objective To investigate the effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats＇ spermatogenic cells. Methods Forty-five 12-month-old male Wistar rats were randomly divided into three groups： young control group（YCG） , natural aging group（NAG） and Heshouwuyin group （SWYG）, 15 rats per group. From 16-month-old, SWYG and NAG were intragastrically given Heshouwuyin and equal amount of normal saline respectively. Gavage treatment kept for 60 days. YCG at 12-month-old and the other two groups at 18-month-old entered experiment respectively. Real-time PCR, immunofluorescenee staining, and Western blotting were used to detect the expression of DR6, BAX, Caspase-3, Cyt-C and 14-3-3ty. Results The expression of 14-3-3ty, a key factor in the mitoehondrial apoptotie pathway, was significantly lower and the expressions of DR6, BAX, Caspase-3, and Cyte were significantly higher in the NAG than in the YCG, but intervention with Heshouwuyin significantly reversed this phenomenon. Conclusion Heshouwuyin improves the spermatogenic function of the testes by regulating expression of key genes in the mitoehondrial apoptosis pathway.