摘要
Objective:To investigate the effect of matrine against biofilm formation of Escherichia coli(E.coli)in the early stage.Methods:Biofilms of E.coli(ATCC25922)was induced by using a peritoneal dialysis catheter in 96-well plates.After treatment with matrine for 24 h,the formation of biofilm on the catheters was observed by scanning electron microscope.The expressions of the flagellar gene(fliC)and motility genes(motA and motB)were determined by qPCR,and the protein expression of fliC was detected by western blotting.Results:The biofilm formation of E.coli was suppressed by different concentrations(less than the minimal inhibitory concentration)of matrine,and the mRNA levels of motA and motB as well as the mRNA and protein expression levels of fliC were decreased in a dose-dependent manner.Conclusion:Matrine could inhibit biofilm formation of E.coli via downregulating the expression of motA,motB and fliC.
Objective： To investigate the effect of matrine against biofilm formation of Escherichia coli （E. coli） in the early stage. Methods： Biofilms of E. coli （ATCC25922） was induced by using a peritoneal dialysis catheter in 96-well plates. After treatment with matrine for 24 h, the formation of biofilm on the catheters was observed by scanning electron microscope. The expressions of the flagellar gene （fliC） and motility genes （motA and motB） were determined by qPCR, and the protein expression of fliC was detected by western blotting. Results： The biofilm formation of E. coli was suppressed by different concentrations （less than the minimal inhibitory concentration） of matrine, and the mRNA levels of motA and motB as well as the mRNA and protein expression levels of fliC were decreased in a dose-dependent manner. Conclusion： Matrine could inhibit biofilm formation of E. coli via downregulating the expression of motA, motB and fliC.
出处
《广西医科大学学报》
CAS
2017年第6期801-805,共5页
Journal of Guangxi Medical University
基金
supported by the National Natural Science Foundation of China(No. 81360111
No.81660133)
