花生枝叶水煎液对大鼠脑缺血再灌注模型的保护作用及机制研究

Study on protective effect and mechanism of decoction of branches and leaves of Arachis hypogaea L on model of cerebral ischemia-reperfusion injury in rats

目的:探讨落花生枝叶水煎液对大鼠脑缺血再灌注损伤(CI-RI)的潜在神经保护作用及其可能机制。方法:将大鼠随机分为对照组、假手术组、模型组以及落花生枝叶低[50 g/(kg·d)]、中[100 g/(kg·d)]、高剂量[150 g/(kg·d)]组,采用改良线栓法闭塞大脑中动脉(MCAO)制备大鼠局灶性CI-RI模型,通过神经缺损行为评分、脑梗死面积测定以及脑组织丙二醛(MDA)、活性氧类(ROS)、谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)等生化指标检测来评价落花生枝叶水煎液的神经保护作用。结果:与模型组相比,落花生枝叶中、高剂量组能显著减轻神经缺损行为体征评分[分别为(2.27±0.80)、(1.93±0.80)和(1.73±0.80)分]、缩小脑梗死体积[分别为(42.46%±6.31%)、(32.20%±7.87%)和(24.80%±4.91%)],同时降低脑组织MDA[(519.66±54.31)、(445.87±12.05)和(303.98±36.03)nmol/mg蛋白]和ROS含量[(2.84±0.12)、(2.10±0.10)和(1.37±0.04)荧光强度/mg蛋白],提高GPx[(32.47±1.92)(66.50±6.04)和(86.39±6.35)U/mg蛋白]和SOD酶活力[(72.53±11.27)、(165.14±14.05)和(258.62±39.21)U/mg蛋白]。结论:落花生枝叶中、高剂量对大鼠CI-RI有潜在的神经保护作用,其作用机制与抗氧化应激有关。

Objective To investigate the potential neuroprotective effect and mechanism of decoction of the branches and leaves of Arachis hypogaea L on model of cerebral ischemia-reperfusion injury （CI-RI） in rats. Methods Rats were grouped as follows： control, sham operation, model, and low, moderate and high dose [（50, 100 and 150 g/（kg.d）, respectivelyl of decoction of the branches and leaves of Arachis hypogaea L. CI-RI model was established by modified suture occlusion of middle cerebral artery （MCAO）. The neuroprotective effect of decoction of the branches and leaves of A rachis hypogaea L was evaluated by the neurological deficit score, cerebral infarct volume, and levels of malondialdehyde （MDA）, reactive oxygen species （ROS）, glutathione peroxidase （GPx）, superoxide dismutase （SOD） in brain tissue. Results Compared with the model group, groups with moderate and high doses of decoction of the branches and leaves of A rachis hy- pogaea L could reduce both the neurological deficit score [（2.27±0.80）, （1.93±0.80） and（1.73±0.80）, respectively] and cerebral infarct volume [（42.46%±6.31%）, （32.20%±7.87%） and （24.80%±4.91%）, respectively], and decrease the levels of MDA [（519.66±54.31）, （445.87±12.05） and （303.98±36.03） nmol/mg protein, respectively] and ROS [（2.84±0.12）, （2.10±0.10） and （1.37±0.04） fluorescence intensity/mg protein, respectively], increase the activities of GPx [（32.47± 1.92）, （66.50±6.04） and （86.39±6.35） U/mg protein, respectively）] and SOD [（72.53±11.27）, （165.14±14.05） and （258.62±39.21） U/mg protein, respectivelyl in brain tissue. Conclusions Decoction of the branches and leaves of A rat：his hypogaea L has potential neuroprotective effect on model of CI-RI in rats, and the mechanism is correlated with its antioxidative stress effect.