成年牛及胎牛β-磷酸三钙煅烧骨骨块的制备、表征和细胞相容性研究

Preparation and Cytocompatibility of Adult and Fetal Bovine Beta-TCP Calcined Bone Blocks

目的:对比研究焦磷酸钠(Na_4P_2O_7.10H_2O,NP)和磷酸二氢铵(NH_4H_2PO_4,MAP)制备的成年牛及胎牛β-TCP煅烧骨骨块的表征及细胞相容性,以期制备出一种理化性能较好且生物安全性可靠的纯相β-TCP煅烧骨骨块。方法:以XRD衍射分析、EDX能谱分析、电镜扫描、Micro-CT扫描及材料力学性能检测研究煅烧骨骨块的表征,以体外细胞毒性实验及细胞与材料的复合实验探究煅烧骨骨块的细胞相容性。结果:NH_4H_2PO_4制备的两种煅烧骨骨块其β-TCP纯度较高,Ca/P比值也均接近于1.47;胎牛煅烧骨骨块的孔隙率、抗压强度及抗弯曲强度均高于成年牛煅烧骨骨块(P<0.05);NH_4H_2PO_4制备的两种煅烧骨骨块细胞毒性均为0级,但胎牛煅烧骨骨块上细胞的粘附与增殖速率高于成年牛煅烧骨骨块。结论:NH_4H_2PO_4制备的胎牛β-TCP煅烧骨骨块具有较好的理化性能及细胞相容性,较适用于非承重区骨缺损的修复。

Objective：To prepare a kind of bone graft material with higher purity onβ-TCP,better properties and perfect cytocompatibility according to the representation and biocompatibility of adult and fetal bovine’s β-TCP calcined bone blocks prepared with NP and MAP.Methods：XRD diffraction analysis,EDX spectrum analysis,electron microscope scanning,Micro-CT scanning,and universal material testing machine were used to compare the properties of two bone blocks.The in vitro cytotoxicity-test and cell-to-material adhesion experiment were used to explore the cytocompatibility.Results：The main ingredients of calcined bone blocks prepared with MAP were similar to pure phaseβ-TCP,and their Ca/P ratio were close to 1.47.The fetal bovine calcined bone blocks’ porosity,compressive strength,and bending strength were higher than those of adult cattle（P〈0.05）.Two kinds of calcined bone blocks’ cytotoxicity were 0levels,but cell adhesion and proliferation rate on fetal bovine bone blocks were higher than those on adult cattle calcined bone blocks.Conclusion：The fetal bovine calcined bone blocks prepared by MAP have better mechanical properties and cytocompatibility,and they are more suitable to apply for bone defect repair in the non-bearing area.