母鼠金雀异黄素干预对雄性子代大鼠肥胖的影响

Effect of maternal genistein intervention on obesity of male offspring rats

[目的]研究母鼠金雀异黄素干预对雄性子代大鼠肥胖的影响。[方法]选用性成熟雌性大鼠,受孕后在孕期及哺乳期间均给予不同剂量的金雀异黄素干预,并设立对照组。干预组母鼠采用添加金雀异黄素的饲料喂饲,剂量分别为3 600、1 200、400 mg/kg。子鼠断乳后采用未添加金雀异黄素的饲料喂饲,直至8周末。实验期间每周观察雄性子鼠的体重;实验结束后测定大鼠身长计算Lee's指数,留取脂肪组织,计算脂肪脏器系数,分离血清进行血脂、血糖、胰岛素和炎性因子的检测。[结果]实验8周末,与对照组相比:3 600 mg/kg组子鼠体重降低(P=0.028)、高密度脂蛋白升高(P=0.038),3 600 mg/kg组和1 200 mg/kg子鼠组Lee's指数、总脂肪湿重、血糖水平降低(P=0.031,P=0.049;P=0.009,P=0.016;P=0.033,P=0.026)。实验组子鼠胰岛素、炎性因子未发生明显变化。[结论]孕期及哺乳期金雀异黄素干预,可以降低雄性子鼠肥胖的发生。

[Objective] To examine the potential effect of maternal genistein intervention on obesity of male offspring rats.[Methods] Mature female rats were given different doses of genistein （3 600, 1 200, and 400 mg/kg feed, respectively） during pregnancy and lactation. A control group fed without genistein was set up. Offspring rats after weaning were fed no-genistein diet for eight weeks. The body weight of male offspring rats was observed once a week during the experiment. Lee＇s index and ratio of fat/body weight were calculated after the experiment. Blood lipid, blood glucose, insulin, and inflammatory factors in serum samples were detected.[Results] After eight weeks, compared with the control group, the male offspring rats following maternal 3 600 mg/kg genistein treatment showed reduced body weight （P=0.028） and increased high-density lipoprotein cholesterol （P=0.038）; those following maternal 3 600 mg/kg and 1 200 mg/kg genistein treatment showed decreased Lee＇s index, total wet weight of fat, and blood glucose （P=0.031, P=0.049; P=0.009, P=0.016; P=0.033, P=0.026）. No obvious changes in insulin and cytokines were found in the offspring rats with maternal genistein treatment.[Conclusion] Maternal genistein intake during pregnancy and lactation could reduce the occurrence of offspring obesity.