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新疆四种土鸡中PRL 24 bp基因插入缺失与产蛋性能相关性分析

Correlation analysis between 24 bp PRL gene insertion deletion and egg production performance in four kinds of chicken from Xinjiang
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摘要 为了研究PRL启动子一个24 bp的插入缺失突变在南疆昆仑山脉四个地域分布的阿合奇土鸡、拜城油鸡、和静土鸡和塔什库尔干土鸡中的多态性及其与产蛋性能之间的联系,试验对四种土鸡500日龄产蛋数进行统计分析,对PRL 24 bp进行直接测序分析。结果表明:在新疆昆仑山脉分布的这四个地方土鸡500日龄产蛋数量之间有显著差异,阿合奇土鸡产蛋性能显著高于其他土鸡。在阿合奇土鸡中ID型的500日龄产蛋量高于DD型,比例为158∶150,但二者之间差异不显著(P=0.169 0),其他鸡种的DD和ID型产蛋数较为接近。说明四种土鸡中均存在24 bp插入缺失的多态性,四种土鸡的基因型频率有显著差异,以DD型和ID型基因型为主要基因型。 The aim of the current study was to investigate the PRL promoter of a 24 bp insertion deletion in the pmlactin promoter and estimate their effects on egg production trait in chickens from Kunlun Mountains, Xinjiang, including geographic area of Aheqi, Hejing, Baicheng and Taxkorgan. The egg production was analyzed in the four local chicken of 500 days of age through sequence analysis of 24 bp PRL. Results showed that there was a significant difference among egg production of the four local chicken of 500 days of age from Kunlun Mountains of Xinjiang distri- bution; the Aheqi chicken egg laying performance was significantly higher than that of other chicken; in Aheqi chicken ID type of 500 days old, egg production was higher than that of DD genotype ( 158:150), but they had no significant difference ( P = 0.169 0). In other chickens, the num- ber of DD and ID type of egg laying had no significant difference. These data indicated the 24 bp insertion deletion polymorphic exist in the four local chicken, four local chicken genotypes were significantly different, DD and ID the main genetic type for the four kinds of chicken.
出处 《黑龙江畜牧兽医》 CAS 北大核心 2017年第6期38-40,48,共4页 Heilongjiang Animal Science And veterinary Medicine
基金 国家自然科学基金项目"新疆南疆部分山区地方品种鸡遗传多样性及生产性能相关分析""新疆和田地区家鸡群体遗传多样性 起源和保护研究"(31060294 3116048) 新疆生产建设兵团塔里木畜牧科技重点实验室开放课题"和田地区地方品种鸡资源调查及种质特性比较研究""利用STR分型技术检测安集延鸡 吐鲁番斗鸡及喀什地区斗鸡之间的遗传距离"(HS201103 HS2014013)
关键词 催乳素(PRL) 24bp 土鸡 产蛋性能 相关性 插入缺失 PRL 24 bp chicken laying performance correlation insertion deletion
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