正交试验优化提取壮药马尾千金草生物碱

Optimizing the Extraction of Alkaloids from Lyeopodium sieboldii Miq by Orthogonal Design

[目的]优化马尾千金草生物碱的提取条件。[方法]以马尾千金草的总生物碱含量为指标,运用正交试验设计对酸水浓度、料液比、浸提次数3个因素进行考查,优化最佳提取工艺。[结果]酸水浸泡提取的最佳条件为加入10倍的1%酸水液,浸泡提取3次,每次24 h,生物碱得率为0.0751%,马尾千金草生物碱含量为7.51 mg/g。[结论]该提取方法操作较简单、成本低、生物碱得率高,能有效提取马尾千金草总生物碱。

[ Objective ] The research aimed to optimize the extraction condition of alkaloids from Lyeopodium sieboldii. [ Method ] Taking the total alkaloid content of Lyeopodium sieboldii as index,the orthogonal experiment was used to evaluate acid water concentration, solid-liquid ratio and extraction times. The optimum extraction process was optimized. [ Result ] The optimum condition of the acid water extraction was 1% acid water solution with 10 times, soaking and extracting for 3 times, each time 24 h, the total alkaloids was 0. 0751%, the alkaloid content of Lyeopodium sieboldii was 7.51 mg/g. [ Conclusion] The extraction method is simple,low operation cost, high rate of alkaloids, which can effectively extract the total alkaloids of Lyeopodium sieboldii.