脂肪酸单甘油酯的酶催化合成及其表征

Enzymatic synthesis and characterization of monoglycerides

以癸酸甲酯、月桂酸甲酯、肉豆蔻酸甲酯、棕榈酸甲酯和硬脂酸甲酯为原料,与甘油进行脂肪酶催化酯交换反应制备脂肪酸单甘油酯,产物为脂肪酸单甘油酯和少量脂肪酸双甘油酯的混合物,其中脂肪酸单甘油酯的质量分数均在91%以上,最高可达95.9%。随着碳链长度的增加,脂肪酸甲酯的转化率降低,脂肪酸单甘油酯的质量分数也降低。采用气相色谱-质谱联机、核磁共振氢谱和红外光谱,对柱层析分离后的脂肪酸单甘油酯进行了结构表征。

Monoglycerides were prepared by the transesterification of fatty acid methyl esters such as methyl ca- prate, methyl laurate, methyl myristate, methyl palmitate and methyl stearate with glycerol in the presence of No- vozyme 435 ]ipase as catalyst. The product is a mixture of monoglycerides and a small amount of diglycerides, which contained monoglycerides above 91% , and up to 95.9%. With the increase of carbon chain length, the conversion of fatty acid methyl esters decreased, and the content of monoglycerides in the products also decreased. After being puri- fied by column chromatography, the final products was characterized by Gas chromatography - mass spectrometry （GC-MS） , H nuclear magnetic resonance （ 1H NMR） and infrared spectra （IR）.