响应面优化鱼鳔胶原肽制备工艺及其抗氧化活性研究

Optimization of swimming bladder collagen peptide preparation using response surface methodology and its antioxidant activity research

鱼鳔富含胶原蛋白,营养价值高,但目前对鱼鳔的加工利用不足,造成资源浪费、环境污染。以草鱼鱼鳔为原料,以DPPH自由基清除能力为评价指标,通过单因素和响应面实验优化酶解鱼鳔胶原蛋白制备抗氧化活性肽的最佳工艺,并研究其体外抗氧化活性。结果表明:抗氧化活性肽的最佳制备工艺为,以风味蛋白酶为酶制剂,酶解时间79.34 min,料液比为3.09∶100(g∶m L),酶添加量为6 343.43 U/g,在此条件下制备的鱼鳔胶原肽的DPPH自由基清除能力达79.98%。鱼鳔胶原肽清除DPPH自由基、羟自由基的IC50分别为8.05 mg/m L和3.54 mg/m L,并且具有较强的还原力。因此,鱼鳔胶原肽具有较强的抗氧化活性,草鱼鱼鳔是制备抗氧化活性肽的良好原料。

Swim bladder is rich in collagen, high in nutrition, but it is waste and cause environmental pollution due to lack of processing technology, causing severe waste and environmental pollution. The technology parameters of enzymatic hydrolysis swim bladder in preparing collagen peptide through single factor experiment and response surface methodology was optimized, DPPH·scavenging ability was used as the indicator. The antioxidant activities of pep- tide prepared under the optimal condition were also evaluated. Results indicated that the optimal conditions for prepar- ing antioxidant peptides were： Flavourzyme hydrolyzing for 79.34 min, liquid-to-material of 3.09% , emzyme-colla- gen ratio of 6 343.43 U/g. Under the above conditions, the DPPH ~ scavenging ability of swim bladder collagen pep- tide was 79.98%. The IC50 value for DPPH · and · OH scavenging ability was 8.05 mg/mL and 3.54 mg/mL, re- spectively. The peptide also exhibited relatively strong reducing power. Therefore, swim bladder is a good source in preparing swim bladder collagen with strong antioxidant activity.