双水相萃取法分离纯化金针菇子实体多糖

Isolation and purification of aqueous two-phase extraction of polysaccharides from Flammulina velutipes

根据双水相萃取原理,探讨了乙醇-硫酸铵双水相体系对金针菇多糖的分配途径,并对该体系的双节线数据进行拟合,确定出拟合度高且参数较少的双节线方程:y=-5.83-64.76×0.95x,R^2=0.999 1。以金针菇多糖萃取率为指标,研究系线长度、硫酸铵质量分数、乙醇质量分数对金针菇多糖的萃取效果。结果表明:在乙醇质量分数为24%、(NH_4)_2SO_4质量分数为21%时,系线长度为47.36,多糖在下相的分配系数为10.57,多糖的萃取率可达92.24%。该法简单高效,可用于金针菇多糖的分离纯化。

This study investigated the effect of ethanol-ammonium sulfate aqueous two phase system on the extraction and purification of polysaccharides from Flammtdiaa velutipes based on two aquous phase extraction： The experimental date of this system was simulated and the math model was established as y = - 5.83 - 64.76 ×0.95^x,R^2=0.999 1. The effect of tie line length（TLL） , concentrations of ethanol and ammonium sulfate on the ex- traction yield of polysaccharides from Flammulina velutipes was studied. The results showed the partition coefficient in lower phase and extraction yield of polysaccharides were 10.57 and 92.24% , respectively. The results showed that TLL was 47.36 when ethanol was 24% and （NH4）2SO4 was 21%. This method is simple and efficient and can be used for the isolation and purification of polysaccharides from Flammulina velutipes.