摘要
目的:建立HPLC-ELSD法测定黄芪注射液中单糖和蔗糖含量。方法:色谱柱为Alltech Prevail Carbonhydrate Polymeric NH_2(聚合物氨基柱)(250mm×4.6mm,5μm),以水-乙腈为流动相梯度洗脱,流速为1.0mL/min,检测器为蒸发光散射检测器,漂移管温度为80℃,气体流速为2.0L/min,柱温为35℃。结果:该条件下D-木糖、D-果糖、D-葡萄糖和蔗糖线性范围分别为0.26~2.60mg/min,0.28~2.80mg/min,0.14~1.40mg/min与1.40~14.00mg/min;平均回收率均在95.0%~105.0%范围内,RSD均<2.0%。结论:建立的方法可靠、简单、重复性好,可用于同时测定黄芪注射液中单糖和蔗糖含量,为黄芪注射液的质量控制提供参考。
Objective ： To establish an HPLC-ELSD method for determination of monosaccharide and sucrose components simultaneously in Astragalus injection. Methods：Chromatographic separation was performed on an Alltech Prevail Carbonhydrate Polymeric NH2 column（250mm× 4.6mm, 5μm） using a mobile phase composed of a mixture of acetonitrile and water with gradient elution at a flow rate of 1.0mL/min. Detector was ELSD and column temperature was 35 ℃. The ELSD drift tube temperature was 80 ℃ and the flow rate of the gas was 2.0mL/min. Results：Under the above conditions, the linearity ranges of D-xylose, D-fructose, D-glu- cose, and sucrose were within 0.26-2.60mL/min, 0.28~2.80mL/min, 0. 14-1.40mL/min and 1.40-14.00 mL/min,respectively. The average recoveries were all within 95.0 % - 10.05 %, RSD were less than 2.0 %. Conclusion：This method is reliable, simple and reproducible, it is suitable for the determination of monosaccharide and sucrose in Astragalusinjection, which can provide reference of the quality control for Astragaius injection.
出处
《亚太传统医药》
2017年第10期25-27,共3页
Asia-Pacific Traditional Medicine
