NKT细胞激活剂α-Galcer对小鼠红白血病的抑制作用

Inhibitory effect of NKT cell stimulatorα-Galcer on development of erythroleukemia

(1)目的采用尾静脉注射制备小鼠红白血病模型,探讨α-半乳糖苷神经酰胺(α-Galcer)对红白血病发生和发展的影响。(2)方法体外培养小鼠红白血病细胞系FBL-3,经尾静脉注射接种C57BL/6小鼠。将FBL-3接种小鼠进一步分为α-Galcer处理组和溶剂对照处理组,于FBL-3接种当天分别注射α-Galcer和溶剂DMSO.观察不同处理组FBL-3细胞接种小鼠总体变化,并于FBL-3接种后第8周处死小鼠,观察外周血血象变化和肿瘤结节形成情况。(3)结果与正常小鼠相比,FBL-3细胞接种小鼠实验期间皮肤感染增加。外周血有核红细胞增加,并且出现明显的实体器官肿瘤结节,而α-Galcer处理显著抑制实体器官肿瘤结节的形成。(4)结论通过FBL-3细胞尾静脉注射初步建立红白血病肿瘤小鼠模型,α-Galcer处理对小鼠白血病的发展发挥抑制作用。

Objective To prepare erythroleukemia mouse model through tail vein injection of FBL-3 cell and investigate the effect of a- Galcer on the occurrence and development of erythroleukemia.Methods FBL-3 erythroleukemia cells were cultured in vitro and C57BL/6 erythroleukemia mouse models were prepared by tail vein injection of FBL-3 cells. The FBL-3 injected mice were further divided into two groups by injecting a- Galcerand solvent DMSO respectively on the date of FBL- 3 injection. The overall changes of FBL-3 injected mice in each group were observed during this experiment. 8 weeks after FBL-3 injection, the FBL-3 injected mice were killed and peripheral blood and tumor nodules formation were detected. Results Compared with normal C57BL/6 mice, the FBL-3 injected mice demonstrated obvious skin infection, increased nucleated red blood cells and apparent tumor nodules formation, whilea- Galcer treatment significantly inhibited the formation of tumor nodules on solid organs. Conclusion Erythroleukemia mouse models were prepared preliminarily by tail intravenous injection of FBL-3 cells, and a- Galcer treatment exhibited an inhibitory effect on the development of leukemia in mice.