产紫杉醇内生真菌和食用菌液体发酵多糖含量比较研究

Comparison analysis of liquid fermentation of polysaccharide content in taxol production in the fungi and edible fungi

用硫酸-苯酚法测定了产紫杉醇内生真菌和2种常见食用菌菌株液体发酵多糖的含量。结果表明:胞外多糖的含量从高到低为:产紫杉醇菌株XX-13>白灵菇4号>产紫杉醇菌株XX-52>白灵菇2号>香菇8808>香菇9319;胞内多糖的含量从高到低为:产紫杉醇菌株XX-13>白灵菇2号>白灵菇4号=香菇8808>香菇9319>产紫杉醇菌株XX-52;总多糖的含量从高到低为:产紫杉醇菌株XX-13>白灵菇2>白灵菇4号>产紫杉醇菌株XX-52>香菇9319>香菇8808。产紫杉醇菌株XX-13液体发酵多糖含量比食用菌的多糖含量高。

The polysaccharide fermented liquid contents of taxol production in the fungi and two common strains of edible fungi were determined by phenol-sulfuric acid method. The results showed that comparison of the exopolysaccharide contents from high to low were taxol production strains XX-13〉Bailing mushroom on the 4th〉taxol production strains XX-52〉Bailing mushroom on the 2nd〉mushrooms 8808〉mushrooms 9319; Comparison of the intracellular polysaccharide contents were taxol production strains XX-13〉Bailing mushroom on the 2nd〉Bailing mushroom on the 4th = 8808〉mushrooms 9319〉taxol production strains XX-52; Comparison of the total polysaccharide were taxol production strains XX-13〉Bailing mushroom 2nd〉Bailing mushroom on the 4th〉taxol production strains XX-52〉mushrooms 9319〉mushrooms 8808. The polysaccharJde contents of taxol production strains XX-13 were higher than that of edible fungi.