黑米花色苷磷脂复合物的制备及生物利用度

Preparation and bioavailability of black rice anthocyanin phospholipid complex

以黑米花色苷和大豆卵磷脂为原料制备黑米花色苷磷脂复合物,以复合率为评价指标,采用单因素和正交实验优化磷脂复合物的最佳制备工艺;测定黑米花色苷及其磷脂复合物在水中和正辛醇中的表观溶解度和油水分配系数;2组大鼠分别灌胃同花色苷含量的黑米花色苷和磷脂复合物,不同时间点眼眶取血,分别绘制血药浓度-时间曲线,并代入3P97代谢动力学软件计算代谢动力学参数,比较生物利用度。结果表明:与黑米花色苷相比,黑米花色苷磷脂复合物在水中的溶解度提高了294倍,在正辛醇中的溶解度降低了399倍,在pH2.5~pH6.5范围内油水分配系数提高了(2~3)倍。大鼠体内实验表明,黑米花色苷血清动力学过程的房室拟合结果倾向于一室模型。黑米花色苷溶液组和磷脂复合物组C(max)分别为0.723、0.919μg/mL,T(peak)分别为1.959、2.117h,AUC分别为4.553、6.634μg/mL·h,磷脂复合物延长了黑米花色苷在体内的半衰期,提高了最大血药浓度和生物利用度。

The black rice anthocyanin phospholipid complex was prepared by using black rice anthocyanin and soybean lecithin as raw materials, and the optimum preparation process was optimized by single factor and orthogonal test. The apparent solubility and water-oil partition coefficients of anthocyanins and their phospholipids complexes in water and n-octanol were determined. The two groups of rats were fed with the same dose of anthocyanin and phospholipid complex of black rice, the orbital blood at different time points were drawn blood concentration-time curve, and put into the 3P97 metabolic kinetics of metabolic kinetic parameters, compared bioavailability degree. The results showed that the solubility of anthocyanin complex in black rice was 294 times higher than that of anthocyanin in black rice, and the solubility in n-octanol was reduced by 399 times. The oil-water partition coefficient was increased 2 to 3 times in the range of pH 2.5-pH 6.5. In vivo experiments of rats showed that the atrioventricular fitting of anthocyanins in black rice tended to be a one-compartment model. The C（max） of black rice anthocyanin group and phospholipid complex group were 0.723μg/mL and 0.919 μg/mL. The T（peak） were 1.959 h and 2.117 h. The AUC were 4.553, 6.634 μg/mLoh. The phospholipid complex prolongs the half-life of anthocyanins in the gastrointestinal tract and increases the maximum plasma concentration and bioavailability.